Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages
| Autor: | Alexander L. Rusanov, Victor G. Zgoda, Jan Sterba, Nataliya G. Luzgina, Dmitry S. Loginov, Martin Selinger, Adéla Chlastáková, Peter M. Kozhin, Libor Grubhoffer, O. V. Tikhonova |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
Proteomics 0301 basic medicine Proteome QH301-705.5 Phagocytosis Down-Regulation PMJ2-R Biology Article Catalysis Inorganic Chemistry 03 medical and health sciences 0302 clinical medicine Animals Macrophage Protein Interaction Maps LC-MS/MS Biology (General) Physical and Theoretical Chemistry HSPA8 QD1-999 Molecular Biology Cells Cultured Spectroscopy Organic Chemistry General Medicine In vitro Up-Regulation Computer Science Applications Cell biology Mice Inbred C57BL Chemistry Gene Ontology 030104 developmental biology ELMO1 Cell culture 030220 oncology & carcinogenesis Macrophages Peritoneal peritoneal macrophages Immortalised cell line |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 6323, p 6323 (2021) International Journal of Molecular Sciences Volume 22 Issue 12 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22126323 |
| Popis: | In vitro models are often used for studying macrophage functions, including the process of phagocytosis. The application of primary macrophages has limitations associated with the individual characteristics of animals, which can lead to insufficient standardization and higher variability of the obtained results. Immortalized cell lines do not have these disadvantages, but their responses to various signals can differ from those of the living organism. In the present study, a comparative proteomic analysis of immortalized PMJ2-R cell line and primary peritoneal macrophages isolated from C57BL/6 mice was performed. A total of 4005 proteins were identified, of which 797 were quantified. Obtained results indicate significant differences in the abundances of many proteins, including essential proteins associated with the process of phagocytosis, such as Elmo1, Gsn, Hspa8, Itgb1, Ncf2, Rac2, Rack1, Sirpa, Sod1, C3, and Msr1. These findings indicate that outcomes of studies utilizing PMJ2-R cells as a model of peritoneal macrophages should be carefully validated. All MS data are deposited in ProteomeXchange with the identifier PXD022133. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|