Optimization of Bioprocess Extraction of Poria cocos Polysaccharide (PCP) with Aspergillus niger β-Glucanase and the Evaluation of PCP Antioxidant Property
| Autor: | Tan Hongyuan, Shiming Li, Peng Wu, Zhan Jianfeng, Ting Hu, Weixin Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Poria cocos polysaccharide
Antioxidant medicine.medical_treatment Pharmaceutical Science antioxidant activity 02 engineering and technology Polysaccharide 01 natural sciences response surface method Article Antioxidants Analytical Chemistry lcsh:QD241-441 Biotransformation lcsh:Organic chemistry Polysaccharides Drug Discovery medicine Food science Physical and Theoretical Chemistry Bioprocess chemistry.chemical_classification Mushroom biology 010405 organic chemistry Chemistry Organic Chemistry Aspergillus niger Temperature Fungal Polysaccharides Hydrogen-Ion Concentration Glucanase 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences Enzyme Chemistry (miscellaneous) Biocatalysis Molecular Medicine β-glucanase biotransformation 0210 nano-technology optimal processing conditions Wolfiporia |
| Zdroj: | Molecules, Vol 25, Iss 5930, p 5930 (2020) Molecules Volume 25 Issue 24 |
| ISSN: | 1420-3049 |
| Popis: | Poria cocos mushroom is widely used as a food and an herb in East Asian and other countries due to its high nutritional value. Research has demonstrated that Poria cocos polysaccharides (PCP) are the major bioactives and possess antioxidation, anti-inflammation, immunoregulation, and other health promoting properties. However, the efficient preparation of PCP has been a challenge, particularly in large scale for industry. Herein, we investigated the biotransformation of PCP from Poria cocos, catalyzed by &beta glucanase from Aspergillus niger and focused on optimizing the most four influencing parameters: Temperature, time, pH, and enzyme dosage in this study. After numerous optimizations with the assistance of response surface optimization methodology, we have established that the optimal conditions for the biotransformation PCP preparation were as following: Enzymolysis temperature 60 ° C, time 120 min, pH 5.0 and enzyme dose 20 mL. Under these conditions, the extraction yield of PCP reached as high as 12.8%. In addition, the antioxidant activities of PCP were evaluated by reducing power assay and 1,1-diphenyl-2-picryl-hydrazyl, superoxide anion, and hydroxyl radicals scavenging assays. Resulting data showed that PCP presented outstanding antioxidant capacity. Thus, these findings indicate that PCP could be produced as a natural antioxidant for further development. |
| Databáze: | OpenAIRE |
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