Does the BioBLU 0.3f single-use scale to the BioFlo® 320 reuseable bioreactor on a matched volumetric oxygen mass transfer coefficient?
| Autor: | Alison Mason, Josh Smith, Duncan Galbraith, Cillian Paget, Williams Olughu, Steve Ruscoe |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Limiting factor Materials science Chemical Phenomena Scale (ratio) Physiology Oxygen mass transfer Analytical chemistry chemistry.chemical_element 010501 environmental sciences Bioprocess development 01 natural sciences Applied Microbiology and Biotechnology Oxygen Industrial Microbiology Bioreactors 010608 biotechnology Bioreactor Scale down of bioprocesses 0105 earth and related environmental sciences Original Paper Single use Bioreactor characterization General Medicine Models Theoretical Scale up of fermentation chemistry Fermentation Biotechnology |
| Zdroj: | World Journal of Microbiology & Biotechnology |
| ISSN: | 1573-0972 0959-3993 |
| Popis: | The volumetric oxygen mass transfer coefficient ($$k_{l} a$$ k l a ) is an essential parameter in aerobic high-cell density fermentation where the availability of oxygen to growing microorganisms is a limiting factor. Bioprocess teams looking to scale-up/down between the Eppendorf BioBLU 0.3f single-use vessel and the BioFlo® 320 reusable vessel bioreactors may find it challenging using a matched $$k_{l} a$$ k l a . The maximum $$k_{l} a$$ k l a of the BioFlo® 320 reusable bioreactor was 109 h−1, which was approximately twice that of the BioBLU 0.3f single-use vessel. The results here show no overlap in $$k_{l} a$$ k l a values when both bioreactors were compared and thus conclude that scalability based on $$k_{l} a$$ k l a is not viable. The maximum $$k_{l} a$$ k l a of the Eppendorf BioBLU 0.3f single-use reported here was 47 h−1 compared to that of the manufacturer’s value of 2500 h−1, indicating a 53-fold difference. This discrepancy was attributed to the incompatible sulfite addition method used by the manufacturer for estimation. |
| Databáze: | OpenAIRE |
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