Effect of RNA interference of the expression of HMGA2 on the proliferation and invasion ability of ACHN renal cell carcinoma cells
| Autor: | Yi‑Yun Wang, Zi‑Ming Wang, Qi‑Zhong Fu, Ying Liu, Ling‑Ling Song, Lin Pu, Zhen‑Long Wang, Jing Liu |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
renal cell carcinoma Cancer Research proliferation Cell Biology Biochemistry 03 medical and health sciences RNA interference 0302 clinical medicine Cell Movement Cell Line Tumor Genetics medicine Humans RNA Messenger RNA Small Interfering Carcinoma Renal Cell Molecular Biology Cell Proliferation Oncogene HMGA2 Protein Articles Transfection Cell cycle invasion Kidney Neoplasms Gene Expression Regulation Neoplastic 030104 developmental biology medicine.anatomical_structure Oncology Cell culture Apoptosis 030220 oncology & carcinogenesis Cancer research Molecular Medicine high mobility group AT-hook 2 A431 cells |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2017.7258 |
| Popis: | This aim of the present study was to observe the effect of high mobility group AT-hook 2 (HMGA2) on the proliferation and invasion ability of ACHN renal cell carcinoma (RCC) cells. Human ACHN cells, an RCC cell line, and HKC normal human renal tubular epithelial cells were cultured. HMGA2 small interfering (si)RNA, Mock-siRNA and their negative control group were designed and synthesized. Subsequently, the ACHN cells were transiently transfected using RNA interference technology. Finally, the mRNA and protein expression levels of HMGA2 were detected using reverse transcription-polymerase chain reaction and western blot analyses. The proliferation ability of the ACHN cells was determined using MTT, and ACHN cell invasion ability was detected using the Transwell method. The results showed that the mRNA and protein expression levels of HMGA2 in the ACHN cells were considerably higher, compared with those in the HKC cells (P |
| Databáze: | OpenAIRE |
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