Relative and combined effects of selenium, protein deficiency and ethanol on bone
| Autor: | José María González-Pérez, Pedro Abreu-Gonzalez, Franscisco Santolaria-Fernández, M.C. Durán-Castellón, Rosa RosVilamajó, L. Galindo-Martín, J. Viña-Rodríguez, María José de la Vega-Prieto, Emilio González-Reimers |
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| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Antioxidant medicine.medical_treatment Osteoporosis chemistry.chemical_element medicine.disease_cause Biochemistry Bone and Bones Inorganic Chemistry Rats Sprague-Dawley Mice Selenium N-terminal telopeptide Internal medicine Protein Deficiency medicine Animals Humans biology Ethanol Chemistry medicine.disease Diet Rats Trace Elements Osteopenia Oxidative Stress Endocrinology Dietary Supplements Osteocalcin biology.protein Solvents Molecular Medicine Oxidative stress Peroxidase |
| Zdroj: | Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS). 25(2) |
| ISSN: | 1878-3252 |
| Popis: | Some observations suggest that oxidative damage may affect both osteoblastic function and osteoclastic activity in alcohol-mediated bone alterations. Selenium, a potent antioxidant, is decreased in alcoholics. Objective To analyse if the supplementation with selenium may alter bone changes observed in a murine model fed ethanol and/or a 2% protein-containing diet, following the Lieber-deCarli design. Material and method Adult male Sprague–Dawley rats were divided into 8 groups, which received the Lieber-DeCarli control diet, an isocaloric, 36% ethanol-containing diet, an isocaloric, 2% protein-containing diet; and an isocaloric diet containing 2% protein and 36% ethanol diet, and another similar four groups to which selenomethionine (1 mg/kg body weight). After sacrifice (5 weeks later), trabecular bone mass was histomorphometrically assessed, bone and serum selenium were determined by flame atomic absorption spectrophotometry, and serum osteocalcin, insulin growth factor 1 (IGF-1), PTH and telopeptide, by radioimmunoanalysis. Liver glutathione peroxidase (GPX) activity was also determined. Results Ethanol-fed rats showed decreased TBM, IGF-1 and osteocalcin, especially when ethanol was added to a 2%-protein diet. Selenium did not modify at all bone parameters, despite a marked increase in serum selenium and a less pronounced one in bone selenium, and an increase in liver GPX. Conclusion Our results do not support the existence of a beneficial effect of selenium addition on bone changes observed in this murine model treated following the Lieber-deCarli experimental design. |
| Databáze: | OpenAIRE |
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