Aniline Induces Oxidative Stress and Apoptosis of Primary Cultured Hepatocytes
| Autor: | Yong-Hui Wu, Jia Yu, Li Jia, Yue Wang, Shu-Ying Dong, Xiao-Lin Na, Hong Gao, Hong-Wei Dong |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Cell Survival DNA damage Health Toxicology and Mutagenesis lcsh:Medicine medicine.disease_cause Article Rats Sprague-Dawley Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Malondialdehyde medicine Animals Humans aniline Viability assay Cells Cultured Membrane Potential Mitochondrial reactive oxygen species chemistry.chemical_classification Reactive oxygen species Aniline Compounds biology Superoxide Dismutase lcsh:R apoptosis Public Health Environmental and Occupational Health Glutathione Catalase Molecular biology hepatocytes Acetylcysteine Rats Oxidative Stress 030104 developmental biology chemistry Apoptosis 030220 oncology & carcinogenesis Hepatocytes biology.protein Environmental Pollutants Oxidation-Reduction Oxidative stress |
| Zdroj: | International Journal of Environmental Research and Public Health International Journal of Environmental Research and Public Health, Vol 13, Iss 12, p 1188 (2016) International Journal of Environmental Research and Public Health; Volume 13; Issue 12; Pages: 1188 |
| ISSN: | 1660-4601 |
| DOI: | 10.3390/ijerph13121188 |
| Popis: | The toxicity and carcinogenicity of aniline in humans and animals have been well documented. However, the molecular mechanism involved in aniline-induced liver toxicity and carcinogenesis remains unclear. In our research, primary cultured hepatocytes were exposed to aniline (0, 1.25, 2.50, 5.0 and 10.0 μg/mL) for 24 h in the presence or absence of N-acetyl-l-cysteine (NAC). Levels of reactive oxygen species (ROS), malondialdehyde (MDA), and glutathione (GSH), activities of superoxide dismutase (SOD) and catalase (CAT), mitochondrial membrane potential, DNA damage, cell viability, and apoptosis were detected. Levels of ROS and MDA were significantly increased and levels of GSH and CAT, activity of SOD, and mitochondrial membrane potential in hepatocytes were significantly decreased by aniline compared with the negative control group. The tail moment and DNA content of the tail in exposed groups were significantly higher than those in the negative control group. Cell viability was reduced and apoptotic death was induced by aniline in a concentration-dependent manner. The phenomena of ROS generation, oxidative damage, loss of mitochondrial membrane potential, DNA damage and apoptosis could be prevented if ROS inhibitor NAC was added. ROS generation is involved in the loss of mitochondrial membrane potential and DNA injury, which may play a role in aniline-induced apoptosis in hepatocytes. Our study provides insight into the mechanism of aniline-induced toxicity and apoptosis of hepatocytes. |
| Databáze: | OpenAIRE |
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