| Autor: |
Mary Pat Carson, Scott W. Peterson, Mary Ellen Moynahan, V. Lerch, R. Vale |
| Rok vydání: |
1982 |
| Předmět: |
|
| Zdroj: |
Experimental Cell Research. 142:447-451 |
| ISSN: |
0014-4827 |
| Popis: |
A plasma membrane-enriched fraction from 3T3 cells has been detergent-solubilized, and the supernatant of this solubilization was reconstituted into liposomes using soybean lecithin. When these vesicles were added to actively growing cells, cell growth rates were inhibited to levels that were comparable to those observed with the original plasma membranes (at least 50% of maximum growth rates). Liposomes without proteins, or liposomes containing proteins from SV3T3 plasma membranes did not significantly inhibit growth of 3T3 cells. Treatment of the reconstituted vesicles with urea or high concentrations of salt did not eliminate the growth-inhibiting properties of these reconstituted membranes. These results indicate that the specific growth-inhibiting factor in 3T3 cell plasma membranes is a membrane protein that has significant non-polar interactions with the membrane bilayer. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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