Diagnostic accuracy of fresh drooled saliva for SARS-CoV-2 in travelers
| Autor: | Kalaiarasu M. Peariasamy, Muhamad Afiq Mu'iz Arifin, Adleen Azman, Sivasooriar Sivaneson, Ganeswrie Rajasekaram, Martin Giddy, Denisa Khoo Fern Ying, Suresh Kumar Chidambaram, Amira Naziffa Shamsuddin, Afifah Haji Hassan, Muhammad Ashraf Mohd Padzil, Alif Adlan Mohd Thabit, Nelson Nheu, Harvinder Kaur Lakhbeer Singh, Camille Cyncynatus, Pei Xuan Kuan, Mohd Asri Yamin |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
medicine.medical_specialty Saliva Coronavirus disease 2019 (COVID-19) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RT-PCR Statistical difference Diagnostic accuracy Asymptomatic Gastroenterology Article Specimen Handling COVID-19 Testing Nasopharynx Internal medicine medicine Humans Travel medicine saliva SARS-CoV-2 business.industry Public Health Environmental and Occupational Health COVID-19 Ct. value Infectious Diseases diagnostic test aviation CT value medicine.symptom business Viral load |
| Zdroj: | Travel Medicine and Infectious Disease |
| ISSN: | 1477-8939 |
| DOI: | 10.1016/j.tmaid.2021.102144 |
| Popis: | BackgroundThe standard for SARS-CoV-2 diagnosis is RT-PCR from nasopharyngeal or oropharyngeal swabs. Major airports require COVID-19 screening, and saliva has the potential as a substitute specimen for SARS-CoV-2 diagnosis. We investigated the utility of fresh drooled saliva against NPS for COVID-19 screening of travelers. MethodsWe recruited 81 travelers and 15 non-travelers (including ten controls) prospectively within a mean of 3⋅22 days of RT-PCR confirmed COVID-19. Each study participant provided 2 mls of early morning fresh drooled whole saliva separately into a sterile plastic container and GeneFiX™ saliva collection kit. The saliva specimens were processed within 4 h and tested for SARS-CoV-2 genes (E, RdRP, and N2) and the results compared to paired NPS RT-PCR for diagnostic accuracy. ResultsMajority of travellers were asymptomatic (75⋅0%) with a mean age of 34⋅26 years. 77 travelers were RT-PCR positive at the time of hospitalization whilst three travelers had positive contacts. In this group, the detection rate for SARS-CoV-2 with NPS, whole saliva, and GeneFiX™ were comparable (89⋅3%, 50/56; 87⋅8%, 43/49; 89⋅6%, 43/48). Both saliva collection methods were in good agreement (Kappa = 0⋅69). There was no statistical difference between the detection rates of saliva and NPS (p > 0⋅05). Detection was highest for the N2 gene whilst the E gene provided the highest viral load (mean = 27⋅96 to 30⋅10, SD = 3⋅14 to 3⋅85). Saliva specimens have high sensitivity (80⋅4%) and specificity (90⋅0%) with a high positive predictive value of 91⋅8% for SARS-CoV-2 diagnosis. ConclusionSaliva for SARS-CoV-2 screening is a simple accurate technique comparable with NPS RT-PCR. |
| Databáze: | OpenAIRE |
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