Acid phosphatase and acid esterase activity in neoplastic and non-neoplastic lymphoid cells A semiquantitative evaluation related to immunological markers in 112 cases
| Autor: | Peter Hokland, O. Myhre Jensen, Anne Marie Boesen |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Naphthol AS D Esterase Rosette Formation Adolescent Lymphoma T-Lymphocytes Acid Phosphatase Lymphoproliferative disorders Biology Esterase immune system diseases hemic and lymphatic diseases medicine Humans Infectious Mononucleosis Lymphocytes Child B cell Aged chemistry.chemical_classification B-Lymphocytes Leukemia Staining and Labeling Lymphoblast Acid phosphatase Hematology Middle Aged medicine.disease humanities Staining medicine.anatomical_structure Enzyme chemistry Cytochemistry biology.protein Female Carboxylic Ester Hydrolases |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0036-553X |
| DOI: | 10.1111/j.1600-0609.1984.tb01697.x |
| Popis: | Acid phosphatase (AcP) and acid alpha-naphthylacetate esterase (ANAE) were examined in lymphoid cells from 104 patients suffering from various lymphoproliferative disorders and from 8 healthy controls. Enzyme activities were evaluated by means of a scoring system. Scores of AcP and ANAE were higher in normal T cells than non-T cells. In comparison, the activated T cells in infectious mononucleosis showed increased AcP and decreased ANAE reaction. Malignant T lymphoblasts had a distinct granular AcP positivity in contrast to the faint reactivity observed in cALL blasts, whereas ANAE showed negative or weak reaction in both subsets. High scores and distinct staining patterns for both enzymes were found in T CLL and T prolymphocytic leukaemia, clearly different from the weak activities seen in B CLL, B PLL and some B cell lymphomas. The latter, too, could be distinguished by mutual differences in enzyme reactions. High AcP and ANAE scores were also found in hairy cell leukaemia, and the staining patterns together with the tartrate resistance firmly established the diagnosis. Thus, simultaneous determinations of AcP and ANAE can be of great value in the diagnosis of lymphoid malignancies. |
| Databáze: | OpenAIRE |
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