Exercise training restores the cardiac microRNA-1 and -214 levels regulating Ca2+ handling after myocardial infarction
| Autor: | Tiago Fernandes, Lucienne S. Lara, Diego R. Mazzotti, Edilamar Menezes de Oliveira, Valério Garrone Barauna, Vander José das Neves, Stéphano Freitas Soares Melo |
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| Rok vydání: | 2015 |
| Předmět: |
Cardiac function curve
Male medicine.medical_specialty Time Factors Heart disease Myocardial Infarction Infarction chemistry.chemical_element Calcium Sodium-Calcium Exchanger Ventricular Function Left Sarcoplasmic Reticulum Calcium-Transporting ATPases Contractility Internal medicine medicine Animals Myocardial infarction Calcium Signaling Rats Wistar Calcium metabolism Sodium-calcium exchanger business.industry Myocardium Recovery of Function medicine.disease Exercise Therapy Disease Models Animal MicroRNAs chemistry Gene Expression Regulation cardiovascular system Cardiology Commentary Cardiology and Cardiovascular Medicine business |
| Zdroj: | BMC cardiovascular disorders. 15 |
| ISSN: | 1471-2261 |
| Popis: | Impaired cardiomyocyte contractility and calcium handling are hallmarks of left ventricular contractile dysfunction. Exercise training has been used as a remarkable strategy in the treatment of heart disease. The microRNA-1, which targets sodium/calcium exchanger 1 (NCX), and microRNA-214, which targets sarcoplasmic reticulum calcium ATPase-2a (Serca2a), are involved in cardiac function regulation. Thus, the aim of this study was to evaluate the effect of exercise training on cardiac microRNA-1 and −214 expression after myocardial infarction. Wistar rats were randomized into four groups: sedentary sham (S-SHAM), sedentary infarction (S-INF), trained sham (T-SHAM), and trained infarction (T-INF). Exercise training consisted of 60 min/days, 5 days/week for 10 weeks with 3 % of body weight as overload beginning four weeks after myocardial infarction. MicroRNA-1 and −214 expressions were, respectively, decreased (52 %) and increased (54 %) in the S-INF compared to the S-SHAM, while exercise training normalized the expression of these microRNAs. The microRNA targets NCX and Serca-2a protein expression were, respectively, decreased (55 %) and increased (34 %) in the T-INF group compared to the S-INF group. These results suggest that exercise training restores microRNA-1 and −214 expression levels and prevents change in both NCX and Serca-2a protein and gene expressions. Altogether, our data suggest a molecular mechanism to restore ventricular function after exercise training in myocardial infarction rats. |
| Databáze: | OpenAIRE |
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