Matrix metalloproteinase induction by EMMPRIN in experimental focal cerebral ischemia

Autor:	Martin Liebetrau, Dorothe Burggraf, Gerhard F. Hamann, Helge K. Martens, Gabriele Jäger, Nathalie Wunderlich, Martin Dichgans
Rok vydání:	2005
Předmět:	Collagen Type IV Male Pathology medicine.medical_specialty Ischemia Matrix metalloproteinase Basement Membrane Brain Ischemia Extracellular matrix Western blot Antigens CD Cortex (anatomy) Basal ganglia medicine Extracellular Animals Rats Wistar medicine.diagnostic_test Chemistry Microcirculation General Neuroscience Cerebral Infarction medicine.disease Immunohistochemistry Matrix Metalloproteinases Extracellular Matrix Rats Up-Regulation Disease Models Animal medicine.anatomical_structure Matrix Metalloproteinase 9 Reperfusion Injury Basigin Matrix Metalloproteinase 2 Basal lamina
Zdroj:	European Journal of Neuroscience. 22:273-277
ISSN:	1460-9568 0953-816X
DOI:	10.1111/j.1460-9568.2005.04187.x
Popis:	Focal cerebral ischemia leads to the gradual disruption of the extracellular matrix. A key role in the turnover of the extracellular matrix is played by the system of matrix metalloproteinases (MMPs). In this study we describe changes of the MMP inducer protein (EMMPRIN) following experimental cerebral ischemia (induced for 3 h and followed by 24 h reperfusion, suture model) in rats. Extracellular EMMPRIN was measured by Western blot of the ischemic and nonischemic basal ganglia and cortex separately. Compared with the contralateral nonischemic area, the ischemic hemisphere showed a significant increase in EMMPRIN: basal ganglia, 158% +/- 4% (P < 0.05); cortex, 128% +/- 25% (P < 0.05). Immunohistochemistry was used to localize EMMPRIN on cerebral microvessels. EMMPRIN-positive microvascular structures were quantified by automatic morphometric video-imaging analysis and a significant increase in the number of cerebral microvessels staining positive for EMMPRIN in the ischemic basal ganglia was shown. The significant loss of microvascular basal lamina antigen collagen type IV in ischemic cortex and basal ganglia was calculated by Western blot. Measured by gelatin zymography, we demonstrated an MMP-2 and MMP-9 increase in the ischemic brain regions (P < 0.05). For the first time the MMP activation system EMMPRIN was shown to be relevant in cerebral ischemia. These results raise the possibility that the increased expression of EMMPRIN, the increase in MMPs and the damage of the basal lamina following cerebral ischemia are connected and part of a network of related changes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a5444c56ee7e00a996dcec2984a1eb5 https://doi.org/10.1111/j.1460-9568.2005.04187.x Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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