De novo assembly of the Mylia taylorii transcriptome and identification of sesquiterpene synthases
| Autor: | Qinggele Caiyin, Weiguo Li, Zhijun Zhang, Jianjun Qiao, Xiaoguang Yan, Dongmei Liang, Guangrong Zhao, Ma Wenzhang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Hepatophyta
0301 basic medicine Biophysics Sequence assembly Biology Sesquiterpene Biochemistry Transcriptome 03 medical and health sciences chemistry.chemical_compound Biosynthesis Transferases Oils Volatile Amino Acid Sequence KEGG Molecular Biology Phylogeny Plant Proteins 030102 biochemistry & molecular biology Gene Expression Profiling Yeast 030104 developmental biology chemistry Structural composition Biocatalysis Mylia taylorii Sequence Alignment Sesquiterpenes |
| Zdroj: | Archives of Biochemistry and Biophysics. 698:108742 |
| ISSN: | 0003-9861 |
| DOI: | 10.1016/j.abb.2020.108742 |
| Popis: | Mylia taylorii is an ancient nonseed land plant that accumulates various sesquiterpenes with insecticidal and antibacterial activities. Recently, microbial-type sesquiterpene synthases (STSs) with atypical aspartate-rich metal ion binding motifs have been identified in some liverworts. Here, transcriptome analysis of M. taylorii was performed to identify M. taylorii sesquiterpene synthases (MtSTSs) that are potentially involved in sesquiterpene biosynthesis and diversity. A total of 255,669 unigenes were obtained with an average length of 963 bp in the transcriptome data of M. taylorii, among which 148,093 (57.92%) unigenes had BLAST results. Forty-eight unigenes were related to the sesquiterpene backbone biosynthesis according to KEGG annotation. In addition, MtSTS1, MtSTS2 and MtSTS3 identified from putative MtSTSs display sesquiterpene catalytic activities on the basis of functional characterizations in yeast. Interestingly, MtSTSs exhibit a noncanonical metal ion binding motif and the structural composition of a single α-domain, which are features of microbial STSs instead of archetypical plant STSs. This study revealed new microbial-type STS members of nonseed plants, and functionally identified that MtSTSs may contribute to the investigation of the biosynthesis and biological role of sesquiterpenes in M. taylorii. |
| Databáze: | OpenAIRE |
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