Delineation of cellular stages and identification of key proteins for reduction and biotransformation of Se(IV) by Stenotrophomonas bentonitica BII-R7
| Autor: | Béatrice Alpha-Bazin, M. Pinel-Cabello, Virginie Chapon, Jean Armengaud, Mohamed L. Merroun, Miguel A. Ruiz-Fresneda, Catherine Berthomieu |
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| Přispěvatelé: | Universidad de Granada = University of Granada (UGR), Biologie végétale et microbiologie environnementale - UMR7265 (BVME), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Interactions Protéine Métal (IPM), Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Granada [Granada], Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Service de Pharmacologie et Immunoanalyse (SPI), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Selenium nanostructures
Se(IV) reduction Proteomics Environmental Engineering Microbial Se(IV) reduction Health Toxicology and Mutagenesis [SDV]Life Sciences [q-bio] Glutathione reductase 0211 other engineering and technologies chemistry.chemical_element 02 engineering and technology 010501 environmental sciences 01 natural sciences Selenium Microbial Biotransformation Se(0) biotransformation Environmental Chemistry Waste Management and Disposal 0105 earth and related environmental sciences 021110 strategic defence & security studies biology biology.organism_classification Pollution 3. Good health Stenotrophomonas Biodegradation Environmental chemistry Biochemistry Catalase [SDE]Environmental Sciences biology.protein Metalloid Bacteria Peroxidase |
| Zdroj: | Journal of Hazardous Materials Journal of Hazardous Materials, 2021, 418, pp.126150. ⟨10.1016/j.jhazmat.2021.126150⟩ Journal of Hazardous Materials, Elsevier, 2021, 418, pp.126150. ⟨10.1016/j.jhazmat.2021.126150⟩ Digibug. Repositorio Institucional de la Universidad de Granada instname |
| ISSN: | 0304-3894 1873-3336 |
| Popis: | This work was supported by the grants obtained by M.P-C: (FPU 15/04284; "Formacion de Profesorado Universitario") and EST 18/00610 ("Ayudas a la movilidad para estancias breves y traslados temporales") from Spanish Ministry (Ministerio de Universidades) . Funding was also provided by grants CGL2014-59616-R; RTI2018.101548.B.I00 to M.L. M. Funding for open access charge: University of Granada/CBUA. The authors thank Concepcion Hernandez-Castillo and Maria del Mar Abad Ortega for their assistance with microscopy at the University of Granada ("Centro de Instrumentacion Cientifica") . We also thank Dr. Kenneth McCreath for editorial support. The widespread use of selenium (Se) in technological applications (e.g., solar cells and electronic devices) has led to an accumulation of this metalloid in the environment to toxic levels. The newly described bacterial strain Stenotrophomonas bentonitica BII-R7 has been demonstrated to reduce mobile Se(IV) to Se(0)-nanoparticles (Se(0) NPs) and volatile species. Amorphous Se-nanospheres are reported to aggregate to form crystalline nanostructures and trigonal selenium. We investigated the molecular mechanisms underlying the biotransformation of Se(IV) to less toxic forms using differential shotgun proteomics analysis of S. bentonitica BII-R7 grown with or without sodium selenite for three different time-points. Results showed an increase in the abundance of several proteins involved in Se(IV) reduction and stabilization of Se(0)NPs, such as glutathione reductase, in bacteria grown with Se(IV), in addition to many proteins with transport functions, including RND (resistance-nodulationdivision) systems, possibly facilitating Se uptake. Notably proteins involved in oxidative stress defense (e.g., catalase/peroxidase HPI) were also induced by Se exposure. Electron microscopy analyses confirmed the biotransformation of amorphous nanospheres to trigonal Se. Overall, our results highlight the potential of S. bentonitica in reducing the bioavailability of Se, which provides a basis both for the development of bioremediation strategies and the eco-friendly synthesis of biotechnological nanomaterials. Spanish Ministry (Ministerio de Universidades) FPU 15/04284 EST 18/00610 University of Granada/CBUA CGL2014-59616-R RTI2018.101548 |
| Databáze: | OpenAIRE |
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