Artifact-free and high-temporal-resolution in vivo opto-electrophysiology with microLED optoelectrodes
| Autor: | Euisik Yoon, John P. Seymour, Kanghwan Kim, György Buzsáki, Mihály Vöröslakos, Kensall D. Wise |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Materials science Light genetic structures Science General Physics and Astronomy Stimulation Optogenetics Neural circuits General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences 0302 clinical medicine Electromagnetic Fields Biological neural network Animals lcsh:Science Electrodes 030304 developmental biology Capacitive coupling 0303 health sciences Artifact (error) Multidisciplinary business.industry Brain General Chemistry Pulse shaping Electrical and electronic engineering Electrophysiological Phenomena Mice Inbred C57BL Electrophysiology Biosensors Modulation Optoelectronics lcsh:Q business Artifacts Biomedical engineering 030217 neurology & neurosurgery |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | The combination of in vivo extracellular recording and genetic-engineering-assisted optical stimulation is a powerful tool for the study of neuronal circuits. Precise analysis of complex neural circuits requires high-density integration of multiple cellular-size light sources and recording electrodes. However, high-density integration inevitably introduces stimulation artifact. We present minimal-stimulation-artifact (miniSTAR) μLED optoelectrodes that enable effective elimination of stimulation artifact. A multi-metal-layer structure with a shielding layer effectively suppresses capacitive coupling of stimulation signals. A heavily boron-doped silicon substrate silences the photovoltaic effect induced from LED illumination. With transient stimulation pulse shaping, we reduced stimulation artifact on miniSTAR μLED optoelectrodes to below 50 μVpp, much smaller than a typical spike detection threshold, at optical stimulation of >50 mW mm–2 irradiance. We demonstrated high-temporal resolution ( Artifact-free opto-electrophysiology is key for precise modulation and monitoring of individual neurons at high spatio-temporal resolution. The authors present a method for eliminating stimulation artifacts in high-density micro-LED optoelectrodes for accurate functional mapping of local circuits. |
| Databáze: | OpenAIRE |
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