Pre- and postnatal exposure to ambient levels of urban particulate matter (PM(2.5)) affects mice spermatogenesis

Autor:	Adriana M. Pires, Heloisa Maria de Siqueira Bueno, Paulo Hilário Nascimento Saldiva, Thais Mauad, Elizabeth Neves de Melo
Rok vydání:	2011
Předmět:	Male medicine.medical_specialty Offspring Testicular volume Health Toxicology and Mutagenesis Embryonic Development Apoptosis Biology Toxicology Testicular weight Andrology Fetal Development Mice Pregnancy Internal medicine Air Pollution Testis medicine SPERMATID RETENTION Animals Cities Spermatogenesis Cell Proliferation Analysis of Variance Inhalation Exposure Mice Inbred BALB C Reproduction Particulates Spermatids Gonadosomatic Index Endocrinology Prenatal Exposure Delayed Effects Female Particulate Matter
Zdroj:	Inhalation toxicology. 23(4)
ISSN:	1091-7691
Popis:	This work characterizes the effects of ambient levels of urban particulate matter (PM(2.5)) from the city of Sao Paulo on spermatogenesis using mice exposed during the embryo-fetal and/or postnatal phases of development. Parental generations (BALB/c mice) were exposed to air pollution in chambers with or without filtering PM(2.5) for 4 months. Animals were mated, and half of the 1-day-old offspring were moved between chambers, which yielded prenatal and postnatal groups. Remaining offspring comprised the non-exposed and pre+postnatal exposed groups. After 90 days, the animals were sacrificed for testis collection and weighing. Optical microscopy was used for the morphometric analyses of the cell counts, spermatogenic cycle, proliferation, and apoptosis. Prenatally exposed animals presented reduced body and testicular weight with an increased gonadosomatic index (GSI). Testicular volume also decreased, as well as the tubular diameter in testes of the same animals. Proliferation, apoptosis, and spermatogenic cycle analyses showed no significant differences among groups. However, the tubules at stage VII of pre- and postnatal animals presented a reduced number of elongated spermatids. Pre+postnatal group presented higher spermatid head retention at stages VIII-XII. These results show that ambient levels of PM(2.5) from Sao Paulo city affect spermatogenesis by damaging sperm production.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a19ee990ae703a24bd99155b6fdf7fa https://pubmed.ncbi.nlm.nih.gov/21456956 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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