Imaging of intracellular behavior of polymeric nanoparticles in Staphylococcus epidermidis biofilms by slit-scanning confocal Raman microscopy and scanning electron microscopy with energy-dispersive X-ray spectroscopy
| Autor: | Mariko Adachi, Chisato Takahashi, Kusuo Ueno, Junichi Aoyama, Hiromitsu Yamamoto |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Materials science Confocal Nanoparticle Bioengineering Nanotechnology macromolecular substances 02 engineering and technology Biomaterials 03 medical and health sciences symbols.namesake chemistry.chemical_compound Microscopy Staphylococcus epidermidis Lactic Acid Microscopy Confocal Spectrum Analysis X-Rays technology industry and agriculture Biofilm 021001 nanoscience & nanotechnology PLGA 030104 developmental biology chemistry Mechanics of Materials Biofilms Drug delivery Microscopy Electron Scanning symbols Nanoparticles 0210 nano-technology Biological imaging Raman spectroscopy Polyglycolic Acid |
| Zdroj: | Materials Science and Engineering: C. 76:1066-1074 |
| ISSN: | 0928-4931 |
| DOI: | 10.1016/j.msec.2017.03.132 |
| Popis: | In drug delivery systems employing polymeric nanoparticles, accurate delivery of drugs to target sites such as bacterial cells, cell tissues, and organelles is essential. In particular, when designing drug delivery systems for the treatment of the biofilm infections, evaluation of the interaction between polymeric nanoparticles and biofilm or bacterial cells using a simple technique is of significant importance. Here we develop two types of novel techniques for the biological imaging of the intracellular behavior of two types of polymeric nanoparticles, biodegradable chitosan-modified poly (dl-lactide-co-glycolide) (PLGA) nanoparticles and chitosan-modified polyvinyl caprolactam - polyvinyl acetate -polyethylene glycol graft copolymer (Soluplus®, Sol) nanoparticles, within a Staphylococcus epidermidis biofilm. As the first technique, Raman imaging of unstained biological materials using slit-scanning confocal Raman microscopy (unstained Raman imaging) was performed, and as the second, field-emission scanning electron microscopy with energy-dispersive X-ray spectroscopy analysis of biological materials labeled with quantum dots (SEM-QD imaging) was demonstrated. These analyses revealed differing localization of the respective nanoparticles within the biofilm in accordance with the specific interactions of PLGA nanoparticles and Sol nanoparticles with the biofilm. These novel techniques open the door to biological imaging and analyses with high spatial resolution, which will help to understand the efficacy of drug delivery to target materials. |
| Databáze: | OpenAIRE |
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