Role of the SRRz/Rz1 lambdoid lysis cassette in the pathoadaptive evolution of Shigella
| Autor: | Milena Grossi, Bianca Colonna, Martina Pasqua, Gioacchino Micheli, Maria Letizia Di Martino, Adriano Leuzzi, Gianni Prosseda |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Genetics Shigellosis Innate immune system 030106 microbiology Virulence Human pathogen General Medicine Biology medicine.disease_cause medicine.disease Microbiology Bacterial evolution Bacterial-host interaction Pathoadaptive mutation Peptidoglycan Shigella Infectious Diseases 03 medical and health sciences 030104 developmental biology medicine Gene Escherichia coli Pathogen |
| Popis: | Shigella, the etiological agent of bacillary dysentery (shigellosis), is a highly adapted human pathogen. It evolved from an innocuous ancestor resembling the Escherichia coli strain by gain and loss of genes and functions. While the gain process concerns the acquisition of the genetic determinants of virulence, the loss is related to the adaptation of the genome to the new pathogenic status and occurs by pathoadaptive mutation of antivirulence genes. In this study, we highlight that the SRRz/Rz1 lambdoid lysis cassette, even though stably adopted in E. coli K12 by virtue of its beneficial effect on cell physiology, has undergone a significant decay in Shigella. Moreover, we show the antivirulence nature of the SRRz/Rz1 lysis cassette in Shigella. In fact, by restoring the SRRz/Rz1 expression in this pathogen, we observe an increased release of peptidoglycan fragments, causing an unbalance in the fine control exerted by Shigella on host innate immunity and a mitigation of its virulence. This strongly affects the virulence of Shigella and allows to consider the loss of SRRz/Rz1 lysis cassette as another pathoadaptive event in the life of Shigella. |
| Databáze: | OpenAIRE |
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