Impact of Fetal Growth Restriction on the Neonatal Microglial Proteome in the Rat
| Autor: | Olivier Baud, Valérie Faivre, Marie-Laure Possovre, Virginie Salnot, Marit Knoop, Daniel Vaiman, Marina Colella, Andrée Delahaye-Duriez, Johanna Bruce, Jérôme Mairesse, Manuela Zinni, François Guillonneau, Julien Pansiot |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Senescence
proteome microglia Inflammation brain development Biology medicine.disease_cause Article Rats Sprague-Dawley Immune system Downregulation and upregulation IUGR hemic and lymphatic diseases Diet Protein-Restricted medicine Animals Cluster Analysis oxidative stress TX341-641 chemistry.chemical_classification Reactive oxygen species Fetal Growth Retardation Nutrition and Dietetics Microglia Nutrition. Foods and food supply Body Weight Cell biology medicine.anatomical_structure Animals Newborn chemistry inflammation Proteome medicine.symptom Oxidative stress Food Science |
| Zdroj: | Nutrients Volume 13 Issue 11 Nutrients, Vol 13, Iss 3719, p 3719 (2021) |
| ISSN: | 2072-6643 |
| DOI: | 10.3390/nu13113719 |
| Popis: | Microglial activation is a key modulator of brain vulnerability in response to intra-uterine growth restriction (IUGR). However, the consequences of IUGR on microglial development and the microglial proteome are still unknown. We used a model of IUGR induced by a gestational low-protein diet (LPD) in rats. Microglia, isolated from control and growth-restricted animals at P1 and P4, showed significant changes in the proteome between the two groups. The expression of protein sets associated with fetal growth, inflammation, and the immune response were significantly enriched in LPD microglia at P1 and P4. Interestingly, upregulation of protein sets associated with the oxidative stress response and reactive oxygen species production was observed at P4 but not P1. During development, inflammation-associated proteins were upregulated between P1 and P4 in both control and LPD microglia. By contrast, proteins associated with DNA repair and senescence pathways were upregulated in only LPD microglia. Similarly, protein sets involved in protein retrograde transport were significantly downregulated in only LPD microglia. Overall, these data demonstrate significant and multiple effects of LPD-induced IUGR on the developmental program of microglial cells, leading to an abnormal proteome within the first postnatal days. |
| Databáze: | OpenAIRE |
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