Evaluation of Etanercept Treatment in Newborn Rat Model with Hyperoxic Lung Injury

Autor: Aysegul Erdem, Ferhat Cekmez, M. Kursat Fidanci, Turan Tunc, Merih Cetinkaya, Galip Erdem, Guven Kaya, Adem Polat, Mehmet Saldir, Onder Onguru, Yasemin Gulcan Kurt
Rok vydání: 2016
Předmět:
medicine.medical_specialty
Pathology
Acute Lung Injury
Hyperoxia
Lung injury
medicine.disease_cause
Etanercept
Pathology and Forensic Medicine
Rats
Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
030225 pediatrics
Internal medicine
medicine
Animals
chemistry.chemical_classification
Tumor Necrosis Factor-alpha
business.industry
Glutathione peroxidase
Anti-Inflammatory Agents
Non-Steroidal
General Medicine
respiratory system
medicine.disease
Immunohistochemistry
Rats
Vascular endothelial growth factor
Disease Models
Animal
Oxidative Stress
Endocrinology
Animals
Newborn
030228 respiratory system
chemistry
Bronchopulmonary dysplasia
Pediatrics
Perinatology and Child Health
Lamellar body membrane
medicine.symptom
business
Oxidative stress
medicine.drug
Zdroj: Fetal and Pediatric Pathology. 35:327-338
ISSN: 1551-3823
1551-3815
DOI: 10.1080/15513815.2016.1189018
Popis: Many factors contribute to the development of BPD basically by increasing inflammation in preterm lungs. However, premature neonates have insufficient anti-inflammatory capacity. We aimed to evaluate the effect of etanercept, an anti-TNF agent, on BPD development in newborn rat model with hyperoxia-induced lung injury.Thirty-two newborn rats were divided into 3 groups as control group (Group 1, n = 11), hyperoxia + placebo group (Group 2, n = 10), and hyperoxia + etanercept group (Group 3, n = 11). Histopathological and biochemical analysis were performed in order to assess inflammation and oxidative stress. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities, and malondialdehyde (MDA) levels were studied, histopathological scoring and radial alveolar count were applied in lung tissue. Lamellar body membrane protein, vascular endothelial growth factor (VEGF), nuclear factor-kappaB (NF-κB) gene expressions were studied in immunohistochemical evaluation of tissue samples. All three groups were compared with each other in terms of all parameters.SOD and GSH-Px activities were significantly higher, whereas MDA levels were lower in group 3, compared to group 2 (p0.001). Histopathological scores were lower, lamellar body membrane protein expression and radial alveolar count were higher in group 3 (p0.05). NF-κB expression was higher in group 2, but lower in group 3 in comparison with group 1. Expression of VEGF was decreased in group 2 but came close to group 1 with etanercept treatment in group 3.We found etanercept treatment to be protective in newborn rats with hyperoxia-induced lung damage.
Databáze: OpenAIRE
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