Irisin modulates genes associated with severe coronavirus disease (COVID-19) outcome in human subcutaneous adipocytes cell culture

Autor: Lucas Solla Mathias, Célia Regina Nogueira, Miriane de Oliveira, Marna Eliana Sakalem, Bruna Moretto Rodrigues, Maria Teresa De Sibio
Přispěvatelé: Universidade Estadual Paulista (Unesp), Universidade Estadual de Londrina (UEL)
Rok vydání: 2020
Předmět:
0301 basic medicine
Jumonji Domain-Containing Histone Demethylases
viruses
Histone Deacetylase 2
Adipose tissue
Cell Cycle Proteins
medicine.disease_cause
Biochemistry
ADAM10 Protein
0302 clinical medicine
Endocrinology
Sirtuin 1
Adipocytes
Furin
Extracellular structure organization
Cells
Cultured
Histone Acetyltransferases
Coronavirus
Regulation of gene expression
TRIB3
Nuclear Proteins
ADAM10
FURIN
Angiotensin-Converting Enzyme 2
Coronavirus Infections
Signal Transduction
Irisin
Pneumonia
Viral
030209 endocrinology & metabolism
Peptidyl-Dipeptidase A
Protein Serine-Threonine Kinases
Biology
Models
Biological
Article
Betacoronavirus
03 medical and health sciences
medicine
Humans
Obesity
Pandemics
Molecular Biology
Gene
SARS-CoV-2
COVID-19
Membrane Proteins
Molecular Sequence Annotation
Fibronectins
Toll-Like Receptor 3
Repressor Proteins
rab1 GTP-Binding Proteins
Gene Ontology
030104 developmental biology
Gene Expression Regulation
Viral replication
Cell culture
biology.protein
Cancer research
Amyloid Precursor Protein Secretases
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Molecular and Cellular Endocrinology
ISSN: 0303-7207
DOI: 10.1016/j.mce.2020.110917
Popis: Obesity patients are more susceptible to develop COVID-19 severe outcome due to the role of angiotensin-converting enzyme 2 (ACE2) in the viral infection. ACE2 is regulated in the human cells by different genes associated with increased (TLR3, HAT1, HDAC2, KDM5B, SIRT1, RAB1A, FURIN and ADAM10) or decreased (TRIB3) virus replication. RNA-seq data revealed 14857 genes expressed in human subcutaneous adipocytes, including genes mentioned above. Irisin treatment increased by 3-fold the levels of TRIB3 transcript and decreased the levels of other genes. The decrease in FURIN and ADAM10 expression enriched diverse biological processes, including extracellular structure organization. Our results indicate a positive effect of irisin on the expression of multiple genes related to viral infection in the adipose tissue; furthermore, translatable for other tissues and organs targeted by the novel coronavirus and present, thus, promising approaches for the treatment of COVID-19 infection as therapeutic strategy to decrease ACE2 regulatory genes.
Highlights • Genes associated with increased virus, SARS-CoV-2, replication are diminished, whereas, the gene that decreases virus replication is elevated by irisin in human subcutaneous adipocytes. • TRIB3 is increased by irisin. • Irisin diminished FURIN and ADAM10 in human subcutaneous adipocytes.
Databáze: OpenAIRE
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