Involvement of afadin in the formation and remodeling of synapses in the hippocampus
Autor: | Hisayuki Amano, Yoshimi Takai, Tomohiro Yamada, Hideru Togashi, Toshiaki Sakisaka, Miki Tanaka-Okamoto, Hisakazu Ogita, Jun Miyoshi, Takashi Majima, Hiroyoshi Ishizaki |
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Rok vydání: | 2009 |
Předmět: |
Mossy fiber (hippocampus)
Nectins Biophysics Hippocampus Biology Hippocampal formation Biochemistry Mice Nectin medicine Animals Cell adhesion Molecular Biology beta Catenin Mice Knockout Cadherin Pyramidal Cells Microfilament Proteins Histone-Lysine N-Methyltransferase Cell Biology Cadherins Cell biology medicine.anatomical_structure nervous system Synapses Synaptic plasticity Pyramidal cell Cell Adhesion Molecules |
Zdroj: | Biochemical and Biophysical Research Communications. 385:539-544 |
ISSN: | 0006-291X |
Popis: | In the hippocampus, synapses are formed between mossy fiber terminals and CA3 pyramidal cell dendrites and comprise highly developed synaptic junctions (SJs) and puncta adherentia junctions (PAJs). Dynamic remodeling of synapses in the hippocampus is implicated in learning and memory. Components of both the nectin–afadin and cadherin–catenin cell adhesion systems exclusively accumulate at PAJs. We investigated the role of afadin at synapses in mice in which the afadin gene was conditionally inactivated in hippocampal neurons. In these mutant mice, the signals for not only nectins, but also N-cadherin and β-catenin, were hardly detected in the CA3 area, in addition to loss of the signal for afadin, resulting in disruption of PAJs. Ultrastructural analysis revealed an increase in the number of perforated synapses, suggesting the instability of SJs. These results indicate that afadin is involved not only in the assembly of nectins and cadherins at synapses, but also in synaptic remodeling. |
Databáze: | OpenAIRE |
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