A novel fast method for aqueous derivatization of THC, OH-THC and THC-COOH in human whole blood and urine samples for routine forensic analyses

Autor: Federica Giorgia Pesci, Mario Giusiani, Silvio Chericoni, Fabio Stefanelli
Rok vydání: 2017
Předmět:
Analyte
Δ9‐tetrahydrocannabinol
11‐nor‐Δ9‐tetrahydrocannabinol‐carboxylic acid
11‐hydroxy‐Δ9‐tetrahydrocannabinol
11‐nor‐Δ9‐tetrahydrocannabinol‐carboxylic acid
aqueous derivatization
cannabinoids
human blood
human urine
Δ9‐tetrahydrocannabinol
Clinical Biochemistry
Liquid-Liquid Extraction
Urine
aqueous derivatization
030226 pharmacology & pharmacy
01 natural sciences
Biochemistry
Gas Chromatography-Mass Spectrometry
Analytical Chemistry
03 medical and health sciences
chemistry.chemical_compound
cannabinoids
Forensic Toxicology
0302 clinical medicine
human urine
Limit of Detection
mental disorders
Drug Discovery
Trimethyloxonium tetrafluoroborate
Humans
Selected ion monitoring
Dronabinol
human blood
Derivatization
Molecular Biology
Pharmacology
Detection limit
Chromatography
organic chemicals
010401 analytical chemistry
Reproducibility of Results
General Medicine
BSTFA
0104 chemical sciences
11‐hydroxy‐Δ9‐tetrahydrocannabinol
chemistry
Linear Models
Quantitative analysis (chemistry)
Zdroj: Biomedical chromatography : BMC. 32(4)
ISSN: 1099-0801
Popis: A novel aqueous in situ derivatization procedure with propyl chloroformate (PCF) for the simultaneous, quantitative analysis of Δ9-tetrahydrocannabinol (THC), 11-hydroxy-Δ9-tetrahydrocannabinol (OH-THC) and 11-nor-Δ9-tetrahydrocannabinol-carboxylic acid (THC-COOH) in human blood and urine is proposed. Unlike current methods based on the silylating agent (BSTFA) added in an anhydrous environment, this new proposed method allows to add the derivatizing agent (propyl chloroformate, PCF) directly to the deproteinized blood and recover the derivatives by liquid-liquid extraction. This novel method can be also used for hydrolyzed urine samples, which is faster than the traditional method involving a derivatization with trimethyloxonium tetrafluoroborate (TMO). The analytes are separated, detected and quantified by gas chromatography-mass spectrometry (GC-MS) in selected ion monitoring mode (SIM). The method was validated in terms of selectivity, capacity of identification, limits of detection (LOD) and quantification (LOQ), carryover, linearity, intra-assay precision, inter-assay precision and accuracy. The limits of detection (LOD) and quantification (LOQ) in hydrolyzed urine were 0.5 ng/mL and 1.3 ng/mL for THC and 1.2 ng/mL and 2.6 ng/mL for THC-COOH, respectively. In blood, the LOD and LOQ were 0.2 ng/mL and 0.5 ng/mL for THC, 0.2 ng/mL and 0.6 ng/mL for OH-THC, 0.9 ng/mL and 2.4 ng/mL for THC-COOH, respectively. This method was applied to thirty-five urine samples and fifty blood samples resulting to be equivalent to the current ones with the advantage of a more simple and faster sample processing time. We believe it will be a more convenient option for the routine analysis of cannabinoids in toxicological and forensic laboratories.
Databáze: OpenAIRE
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