Toward biomimetic materials in bone regeneration: Functional behavior of mesenchymal stem cells on a broad spectrum of extracellular matrix components
| Autor: | Georg Matziolis, Georg N. Duda, Simone Frauenschuh, Andrea Ode, Carsten Perka, Juliane D. Glaeser, Cameron J. Wilson, Grit Kasper |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Integrins Bone Regeneration Materials science Molecular Sequence Data Biomedical Engineering Matrix (biology) MSC functional behavior Biomaterials Extracellular matrix Biomimetic Materials Cell Movement Osteogenesis Materials Testing 111601 Cell Physiology Cell Adhesion medicine Animals Humans Amino Acid Sequence Bone regeneration Cell Proliferation biology Cartilage Mesenchymal stem cell Metals and Alloys Reproducibility of Results 060106 Cellular Interactions (incl. Adhesion Matrix Cell Wall) Cell Differentiation Mesenchymal Stem Cells Adhesion Extracellular Matrix Cell biology Fibronectin medicine.anatomical_structure 090301 Biomaterials Ceramics and Composites biology.protein Cattle Female Stem cell Peptides Biomarkers Biomedical engineering |
| Zdroj: | Journal of Biomedical Materials Research-Part A |
| ISSN: | 1549-3296 |
| DOI: | 10.1002/jbm.a.32909 |
| Popis: | Bone defect treatments can be augmented by mesenchymal stem cell (MSC) based therapies. MSC interaction with the extracellular matrix (ECM) of the surrounding tissue regulates their functional behavior. Understanding of these specific regulatory mechanisms is essential for the therapeutic stimulation of MSC in vivo. However, these interactions are presently only partially understood. This study examined in parallel, for the first time, the effects on the functional behavior of MSCs of 13 ECM components from bone, cartilage and hematoma compared to a control protein, and hence draws conclusions for rational biomaterial design. ECM components specifically modulated MSC adhesion, migration, proliferation, and osteogenic differentiation, for example, fibronectin facilitated migration, adhesion, and proliferation, but not osteogenic differentiation, whereas fibrinogen enhanced adhesion and proliferation, but not migration. Subsequently, the integrin expression pattern of MSCs was determined and related to the cell behavior on specific ECM components. Finally, on this basis, peptide sequences are reported for the potential stimulation of MSC functions. Based on the results of this study, ECM component coatings could be designed to specifically guide cell functions. |
| Databáze: | OpenAIRE |
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