Enzymatic cross-linking of carboxymethylpullulan grafted with ferulic acid
| Autor: | Didier Le Cerf, Ahdi Hadrich, Luc Picton, Virginie Dulong |
|---|---|
| Přispěvatelé: | Polymères Biopolymères Surfaces (PBS), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2016 |
| Předmět: |
Coumaric Acids
Polymers and Plastics Kinetics 02 engineering and technology 010402 general chemistry 01 natural sciences Ferulic acid Hydrophobic effect chemistry.chemical_compound Elastic Modulus Polymer chemistry Materials Chemistry medicine [CHIM]Chemical Sciences Glucans ComputingMilieux_MISCELLANEOUS Laccase Chemistry Organic Chemistry Hydrogels Pullulan 021001 nanoscience & nanotechnology Grafting 0104 chemical sciences Cross-Linking Reagents [CHIM.POLY]Chemical Sciences/Polymers Proton NMR Swelling medicine.symptom Rheology 0210 nano-technology Nuclear chemistry |
| Zdroj: | Carbohydrate Polymers Carbohydrate Polymers, Elsevier, 2016, 151, pp.78-87. ⟨10.1016/j.carbpol.2016.05.057⟩ |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2016.05.057 |
| Popis: | Carboxymethylpullulan (CMP) has been modified in a two-step grafting reaction of ferulic acid (FA). Acid adipic dihydrazyde (ADH) was first reacted with FA activated with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC). Then the product of this first reaction was reacted with CMP (activated with EDC). Grafted polysaccharides structure was confirmed by FTIR and (1)H NMR spectroscopy. Analyses by size-exclusion chromatography (SEC) coupling on-line with a multi-angle light scattering detector (MALS), a viscometer and a differential refractive index detector (DRI) (SEC/MALS/DRI/Visco) showed that associations between FA moieties occurred due to hydrophobic interactions. The grafting rates of FA were determined by the Folin-Ciocalteu method and were found between 1.0% and 11.2% (mol/mol anhydroglucose unit). The CMP-FA were then enzymatically cross-linked with laccase from Pleurotus ostreatus. The crosslinking reactions were followed by rheological measurements, demonstrating the influence of laccase concentration on kinetics. Elastic modulus and swelling rates of hydrogels depends on FA content only for low values. |
| Databáze: | OpenAIRE |
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