Involvement of hyaluronidases in colorectal cancer
| Autor: | Helen Bouga, Michael S Stavropoulos, N. Papageorgakopoulou, Isidoros Tsouros, Dimitrios A. Theocharis, Dimitrios Bounias, Demitrios H. Vynios, Dora Kyriakopoulou |
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| Rok vydání: | 2009 |
| Předmět: |
Gene isoform
Male Pathology medicine.medical_specialty Cancer Research Colorectal cancer Angiogenesis Colon Blotting Western Hyaluronoglucosaminidase GPI-Linked Proteins lcsh:RC254-282 Hyaluronidase Surgical oncology Genetics Biomarkers Tumor Medicine Humans Zymography RNA Messenger Aged Neoplasm Staging Aged 80 and over business.industry Reverse Transcriptase Polymerase Chain Reaction Rectum Middle Aged medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Prognosis Blot Survival Rate Oncology Cancer research Disease Progression Tumor promotion Female business Colorectal Neoplasms Cell Adhesion Molecules medicine.drug Research Article |
| Zdroj: | BMC Cancer BMC Cancer, Vol 10, Iss 1, p 499 (2010) |
| ISSN: | 1471-2407 |
| Popis: | Background Hyaluronidases belong to a class of enzymes that degrade, predominantly, hyaluronan. These enzymes are known to be involved in physiological and pathological processes, such as tumor growth, infiltration and angiogenesis, but their exact role in tumor promotion or suppression is not clear yet. Advanced colorectal cancer is associated with elevated amounts of hyaluronan of varying size. The aim of the present study was therefore to illuminate the importance of hyaluronidases in colon carcinoma progression. Methods The patients' samples (macroscopically normal and cancerous) were subjected to sequential extraction with PBS, 4 M GdnHCl and 4 M GdnHCl - 1% Triton X-100. The presence of the various hyaluronidases in the extracts was examined by zymography and western blotting. Their expression was also examined by RT-PCR. Results Among hyaluronidases examined, Hyal-1, -2, -3 and PH-20 were detected. Their activity was higher in cancerous samples. Hyal-1 and Hyal-2 were overexpressed in cancerous samples, especially in advanced stages of cancer. Both isoforms were mainly extracted with PBS. Hyal-3 was observed only in the third extract of advanced stages of cancer. PH-20 was abundant in all three extracts of all stages of cancer. The expression of only Hyal-1 and PH-20 was verified by RT-PCR. Conclusion A high association of hyaluronidases in colorectal cancer was observed. Each hyaluronidase presented different tissue distribution, which indicated the implication of certain isoforms in certain cancer stages. The results provided new evidence on the mechanisms involved in the progression of colorectal cancer. |
| Databáze: | OpenAIRE |
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