Confronting molecular rotors and self-quenched dimers as fluorogenic BODIPY systems to probe biotin receptors in cancer cells
| Autor: | Kyong T. Fam, Mayeul Collot, Andrey S. Klymchenko, Lazare Saladin |
|---|---|
| Přispěvatelé: | Laboratoire de Bioimagerie et Pathologies (LBP), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Boron Compounds
Dimer Cell 010402 general chemistry 01 natural sciences Catalysis Cell Line Mice chemistry.chemical_compound Neoplasms Materials Chemistry Biotin receptors medicine Animals Humans Receptors Growth Factor Receptor ComputingMilieux_MISCELLANEOUS Fluorescent Dyes Molecular Structure 010405 organic chemistry Chemistry [CHIM.ORGA]Chemical Sciences/Organic chemistry fungi Metals and Alloys General Chemistry 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials medicine.anatomical_structure Membrane Cancer cell Ceramics and Composites Biophysics Light Up BODIPY |
| Zdroj: | Chemical Communications Chemical Communications, Royal Society of Chemistry, 2021, 57, pp.4807-4810. ⟨10.1039/d1cc00108f⟩ |
| ISSN: | 1359-7345 1364-548X |
| DOI: | 10.1039/d1cc00108f⟩ |
| Popis: | Probing receptors at the cell surface to monitor their expression level can be performed with fluorogenic dyes. Biotin receptors (BRs) are particularly interesting as they are overexpressed in cancer cells. Herein, to image BRs, we adapted and systematically compared two fluorogenic systems based on BODIPYs: a molecular rotor and a self-quenched dimer that light up in response to high viscosity and low polarity of the membrane, respectively. The fluorogenic dimer proved to be more efficient than the rotor and allowed BRs to be imaged in cancer cells, which can effectively be discriminated from non-cancer cells. |
| Databáze: | OpenAIRE |
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