The dopamine mesocorticolimbic pathway is affected by deficiency in n-3 polyunsaturated fatty acids
| Autor: | Denis Guilloteau, Sylvie Chalon, Luc Zimmer, Sylvie Vancassel, Sylvain Cantagrel, Patrick Breton, Séraphin Delamanche, Georges Durand |
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| Přispěvatelé: | Dynamique et pathologie du développement cérébral, Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de nutrition et sécurité alimentaire, Institut National de la Recherche Agronomique (INRA), Centre d'études du Bouchet |
| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty 030309 nutrition & dietetics [SDV]Life Sciences [q-bio] Dopamine Microdialysis Dopamine Agents Medicine (miscellaneous) Nucleus accumbens Biology Mesocortical pathway 03 medical and health sciences 0302 clinical medicine Neurochemical Internal medicine Vesicular Biogenic Amine Transport Proteins medicine Animals Rats Wistar Amphetamine In Situ Hybridization 0303 health sciences Analysis of Variance Neurotransmitter Agents Nutrition and Dietetics Membrane Glycoproteins Dopaminergic Neuropeptides Brain Membrane Transport Proteins Biological Transport Diet Rats Ventral tegmental area Vesicular monoamine transporter medicine.anatomical_structure Endocrinology Vesicular Monoamine Transport Proteins Fatty Acids Unsaturated Female 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Europe PubMed Central American Journal of Clinical Nutrition American Journal of Clinical Nutrition, American Society for Nutrition, 2002, 75 (4), pp.662-667. ⟨10.1093/ajcn/75.4.662⟩ ResearcherID |
| ISSN: | 0002-9165 |
| Popis: | International audience; Background: Several findings in humans support the hypothesis of links between n-3 polyunsaturated fatty acid (PUFA) status and psychiatric diseases. Objective: The involvement of PUFAs in central nervous system function can be assessed with the use of dietary manipulation in animal models. We studied the effects of chronic dietary n-3 PUFA deficiency on mesocorticolimbic dopamine neurotransmission in rats. Design: Using dual-probe microdialysis, we analyzed dopamine release under amphetamine stimulation simultaneously in the frontal cortex and the nucleus accumbens. The messenger RNA (mRNA) expression of vesicular monoamine transporter, and dopamine D, receptor was studied with the use of in situ hybridization. The protein expression of the synthesis-limiting enzyme tyrosine 3-monooxygenase (tyrosine 3-hydroxylase) was studied with the use of immunocytochemistry. Results: Dopamine release was significantly lower in both cerebral areas in n-3 PUFA-deficient rats than in control rats, but this effect was abolished in the frontal cortex and reversed in the nucleus accumbens by reserpine pretreatment, which depletes the dopamine vesicular storage pool. The mRNA expression of vesicular monoamine transporter, was lower in both cerebral areas in n-3 PUFA-deficient rats than in control rats, whereas the mRNA expression of D, receptor was lower in the frontal cortex and higher in the nucleus accumbens in n-3 PUFA-deficient rats than in control rats. Finally, tyrosine 3-monooxygenase immunoreactivity was higher in the ventral tegmental area in n-3 PUFA-deficient rats than in control rats. Conclusions: Our results suggest that the mesolimbic dopamine pathway is more active whereas the mesocortical pathway is less active in n-3 PUFA-deficient rats than in control rats. This provides new neurochemical evidence supporting the effects of n-3 PUFA deficiency on behavior. |
| Databáze: | OpenAIRE |
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