Lung Cells Transplanted to Irradiated Recipients Generate Lymphohematopoietic Progeny
| Autor: | Gina Lauby, Lidia S. Manouilova, Stephen I. Rennard, J. Graham Sharp, Craig Boyer, Shinji Abe |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Aging Pathology medicine.medical_specialty Cell Transplantation Transgene CD3 Green Fluorescent Proteins Clinical Biochemistry Cell Macrophage-1 Antigen Bone Marrow Cells Mice Transgenic Cell Separation Biology Mice Side population Antigens CD Y Chromosome Leukocytes medicine Animals Lung Molecular Biology Total body Cell Biology Hematopoietic Stem Cells Molecular biology Mice Inbred C57BL Luminescent Proteins medicine.anatomical_structure Injections Intravenous biology.protein Leukocyte Common Antigens Cattle Female Bone marrow Biomarkers Whole-Body Irradiation CD8 |
| Zdroj: | American Journal of Respiratory Cell and Molecular Biology. 30:491-499 |
| ISSN: | 1535-4989 1044-1549 |
| DOI: | 10.1165/rcmb.2003-0140oc |
| Popis: | Bone marrow (stem) cells can differentiate into cells in multiple tissues, including lung. Conversely, there are reports that cells of nonhematopoietic tissues (brain, muscle) can give rise to lymphohematopoietic cells. Here we show that the lung contains cells capable of giving rise to lymphohematopoietic cells when transplanted to irradiated recipients. Whole lung cell suspensions, lung side population (SP) cells, and CD45(+/-) lung cells obtained from male transgenic enhanced green fluorescent protein-expressing mice were transplanted intravenously to total body irradiated female mice. Green fluorescent cells were recovered from the circulation and phenotyped for their expression of lymphohematopoietic markers (CD3, CD4, CD8, B220, Gr-1, and Mac-1). Lung SP cells were composed of heterogeneous populations and had less ability to give rise to lymphohematopoietic cells than did bone marrow SP cells. Furthermore, the ability of cells from the lung of aged mice to generate lymphohematopoietic progeny was equivalent to that of cells from young mice. Cells from lung with radioprotective and lymphohematopoietic reconstituting abilities were CD45(+). CD45(+) cells in the lung cells have lymphohematopoietic stem/progenitor cell characteristics, and this has implications for cell or gene therapy applications. |
| Databáze: | OpenAIRE |
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