Genistein inhibits the proliferation of human choriocarcinoma cells via the downregulation of estrogen receptor-α phosphorylation at serine 118
| Autor: | Edi Hartoyo, Hariadi Yuseran, Tatit Nurseta, Handono Kalim |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Confluency
Nutrition and Dietetics Nutrition. Foods and food supply Cell growth medicine.drug_class Endocrinology Diabetes and Metabolism Choriocarcinoma Trophoblast Malignancy Genistein Estrogen receptor Cell cycle medicine.disease chemistry.chemical_compound chemistry Downregulation and upregulation Estrogen medicine Cancer research TX341-641 |
| Zdroj: | Clinical Nutrition Open Science, Vol 35, Iss, Pp 77-85 (2021) |
| ISSN: | 2667-2685 |
| DOI: | 10.1016/j.yclnex.2020.10.001 |
| Popis: | Summary: Background & aims: Choriocarcinoma is a malignant trophoblastic tumor. The phosphorylation of estrogen receptor-α at serine 118 (p-ER-s118) decreases cancer cell proliferation. However, the effect of genistein as a modulator of p-ER-s118 and proliferation of chorioarcinoma cells remains to be understood. This study aims at determining the function of genistein on p-ER-s118 levels and human choriocarcinoma JEG-3 cell proliferation. Methods: After reaching confluency, cells were divided into six groups, the control group (without methyl-piperidino-pyrazole (MPP) pre-treatment and genistein treatment); and groups with cells treated with genistein at concentrations of 0, 10, 25, 50, and 100 μM (cells were pretreated with MPP). Expression of p-ER-s118 and Ki-67 were analyzed using immunocytochemistry. Results: Different doses of genistein decreased p-ER-s118 levels compared to those in the control (p |
| Databáze: | OpenAIRE |
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