25-Hydroxyvitamin D-1-α-hydroxylase in apoliporotein E knockout mice: The role of protecting vascular smooth muscle cell from calcification
| Autor: | Xiaojie He, Zhuwen Yi, Yan Ding, Wei Xiang, Wang Liao |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Vascular smooth muscle Apolipoprotein B Endothelium Myocytes Smooth Muscle Parathyroid hormone Inflammation 030204 cardiovascular system & hematology Calcitriol receptor Muscle Smooth Vascular 03 medical and health sciences Apolipoproteins E 0302 clinical medicine Internal medicine medicine Vitamin D and neurology Animals 25-Hydroxyvitamin D3 1-alpha-Hydroxylase Mice Knockout Pharmacology biology Calcinosis General Medicine Alkaline Phosphatase medicine.disease Rats Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Endocrinology Cytoprotection biology.protein Receptors Calcitriol Calcium lipids (amino acids peptides and proteins) medicine.symptom Reactive Oxygen Species Calcification |
| Zdroj: | Biomedicine & Pharmacotherapy. 88:971-977 |
| ISSN: | 0753-3322 |
| DOI: | 10.1016/j.biopha.2017.01.093 |
| Popis: | Previous publications widely reported that 25-hydroxyvitamin D-1-α-hydroxylase (CYP27B1) regulated the metabolism of 25-hydroxyvitamin D3, which has a close association between altered activity of vitamin D and vascular calcification has been reported in various human diseases, including chronic kidney disease, osteoporosis and atherosclerosis. Vascular calcification is a clinically significant component of atherosclerosis and may be promoted by ROS associated inflammatory. In this study, we evaluated the effect of 25-hydroxyvitamin D-1-α-hydroxylase on the atherosclerosis disease both in apolipoprotein (apo) E-/- mice and wild-type mice. We also isolated endothelial cell (ECs) and vascular smooth muscle cells (VSMCs) in aortic from the wild type mice and apoE-/- mice respectively, then investigated that after parathyroid hormone (PTH) both of the CYP27B1 and vitamin D receptor (VDR) expressions in apoE-/-EC and apoE-/-VSMC were higher than the wide-type EC and VSMCs. However, the increased proliferation and decreased apoptosis have showed in EC and VSMC compared with the cells from apo E-/- mice. Moreover, the index associated with vascular calcification such as intracellular Ca2+ concentration and alkaline phosphatase (ALP) activity have been tested and the result suggested that the levels of the former index have improved in the apoE-/-EC and apoE-/-VSMC. We got similar conclusions under the pre-treatment with 1, 25(OH) 2D3. |
| Databáze: | OpenAIRE |
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