Experience with in Vitro Sperm Penetration Testing in Infertile and Fertile Couples
| Autor: | L. W. Cox, Amanda Elizabeth Makin, Colin D. Matthews |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Male
Infertility media_common.quotation_subject Obstetrics and Gynecology Semen Penetration (firestop) In Vitro Techniques Biology medicine.disease Mucus Andrology Sperm penetration Repeated testing Reproductive Medicine Cervix Mucus Sperm Motility medicine Humans Gross defects Female Infertility Female Ovulation Infertility Male media_common |
| Zdroj: | Fertility and Sterility. 33:187-192 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/s0015-0282(16)44540-1 |
| Popis: | The ability of spermatozoa to penetrate periovulatory cervical mucus was investigated in 132 infertile couples using an in vitro test system. Particular emphasis was placed upon the accurate endocrinologic localization of ovulation time, on the complete testing during this period, and on repeated testing for couples who demonstrated abnormal results. By comparison, the results of similarly performed tests in the conceptional or immediately preconceptional cycle of 63 couples provided the control data. In addition, when penetration was deficient, crossover testing was instituted using optimal semen or cervical mucus. Absent penetration was found in 17 (13%) of the initial cycles tested of the infertile group. However, with the exception of 2 couples who could not be retested, and 3 couples for whom a semen factor was clearly the explanation, all of the remaining 12 couples demonstrated normal migration when tested during subsequent cycles. The results suggested that defective sperm penetration as delineated by this study is an unlikely contributor to the explanation of the long-term infertility of couples. The results also demonstrated how misjudgments of the importance of this area could have arisen, as 61 % of couples tested showed some periovulatory days when penetration was absent. Over-all, 24% of the days tested showed absent migration, even though closely allied days showed normal penetration. Since a spectrum of normal and subnormal semen was used for testing, the influence of semen on optimal mucus conditions could be evaluated. Such testing was not found to be particularly discriminatory between different semen abnormalities, since satisfactory migration could be seen using semen with gross defects. However, severe motility defects of semen appeared to have the most adverse influence. Deficient penetration due to a semen factor explained only 2.3% of the infertility of the 132 couples investigated. |
| Databáze: | OpenAIRE |
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