The DNA of Bacteria of the World Ocean and the Earth in Cosmic Dust at the International Space Station
| Autor: | Oleg Latyshev, I. A. Zlatskiy, N. Y. Kulikova, T. V. Grebennikova, Anton Syroeshkin, M. A. Chichaeva, L. V. Kostina, E. V. Shubralova, A G Yuzhakov, Olesja Eliseeva, O. S. Tsygankov, M.A. Morozova |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
DNA Bacterial Cosmic Dust Article Subject Earth Planet Oceans and Seas 030106 microbiology Bacillus lcsh:Medicine 01 natural sciences lcsh:Technology General Biochemistry Genetics and Molecular Biology DNA sequencing Bacterial genetics Astrobiology 03 medical and health sciences chemistry.chemical_compound Marine bacteriophage RNA Ribosomal 16S 0103 physical sciences Spacecraft lcsh:Science 010303 astronomy & astrophysics Phylogeny General Environmental Science biology Base Sequence lcsh:T lcsh:R General Medicine Ribosomal RNA biology.organism_classification 16S ribosomal RNA chemistry Genes Bacterial lcsh:Q DNA Bacteria Research Article |
| Zdroj: | The Scientific World Journal, Vol 2018 (2018) The Scientific World Journal |
| ISSN: | 2356-6140 |
| Popis: | Cosmic dust samples from the surface of the illuminator of the International Space Station (ISS) were collected by a crew member during his spacewalk. The sampler with tampon in a vacuum container was delivered to the Earth. Washouts from the tampon’s material and the tampon itself were analyzed for the presence of bacterial DNA by the method of nested PCR with primers specific to DNA of the genusMycobacteria, DNA of the strains of capsular bacteriaBacillus, and DNA encoding 16S ribosomal RNA. The results of amplification followed by sequencing and phylogenetic analysis indicated the presence of the bacteria of the genusMycobacteriaand the extreme bacterium of the genusDelftiain the samples of cosmic dust. It was shown that the DNA sequence of one of the bacteria of the genusMycobacteriawas genetically similar to that previously observed in superficial micro layer at the Barents and Kara seas’ coastal zones. The presence of the wild land and marine bacteria DNA on the ISS suggests their possible transfer from the stratosphere into the ionosphere with the ascending branch of the global electric circuit. Alternatively, the wild land and marine bacteria as well as the ISS bacteria may all have an ultimate space origin. |
| Databáze: | OpenAIRE |
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