Modulation of reactive oxygen species activities and H2O2 accumulation during compatible and incompatible tomato-root-knot nematode interactions

Autor: Paola Leonetti, Teresa Bleve-Zacheo, Philippe Castagnone-Sereno, Michel Bongiovanni, M. Teresa Melillo
Přispěvatelé: Istituto per la Protezione delle Piante (IPP), Interactions plantes-microorganismes et santé végétale (IPMSV), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2006
Předmět:
0106 biological sciences
Physiology
Plant Science
01 natural sciences
Plant Roots
Lycopersicon
HOST PLANT INTERACTION
Host-Parasite Interactions
Cell wall
03 medical and health sciences
chemistry.chemical_compound
Solanum lycopersicum
Botany
Root-knot nematode
Animals
Tylenchoidea
ROOT-KNOT NEMATODE
MELOIDOGYNE
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
Reactive oxygen species
biology
Superoxide
food and beverages
Hydrogen Peroxide
biology.organism_classification
Molecular biology
Immunity
Innate
OXIDATIVE BURST
Respiratory burst
[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Nematode
chemistry
VIRULENCE
PLANT DEFENCE MECHANISM
Solanum
Reactive Oxygen Species
010606 plant biology & botany
Zdroj: New Phytologist
New Phytologist, Wiley, 2006, 170 (3), pp.501-512. ⟨10.1111/j.1469-8137.2006.01724.x⟩
ISSN: 0028-646X
1469-8137
Popis: Here, the interaction of Melodoigyne incognita virulent and avirulent pathotypes with susceptible and Mi-resistant tomato (Solanum lycopersicon) has been studied. Significant differences in nematode penetration occurred 2 days postinoculation (dpi) and became stable from 3 dpi onwards. The hypersensitive cell response (HR) in resistant plants prevented the installation of the avirulent pathotype. The virulent pathotype overcame the Mi (nematode) resistance and induced feeding sites in root cells without triggering HR. Reactive oxygen species (ROS), visualized by subcellular reduction of nitroblue tetrazolium, accumulated in nematode penetrated cells. Quantitative analyses with dichlorofluorescein indicated that the oxidative burst occurred very early with both pathotypes, with an enhanced rate in hyper-responsive cells. Hydrogen peroxide (H(2)O(2)), detected by cerium chloride reaction, accumulated in the cell walls and especially in cells neighbouring HR. The apoplastic location of cerium perhydroxide indicated that either the plasma membrane or the cell wall was the primary site of the superoxide/H(2)O(2) generator. The data provide evidence, for the first time, for ROS-generated signals and their spatiotemporal expression in the host and nonhost interaction of tomato with nematodes.
Databáze: OpenAIRE
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