Possible role of the transcription factor interferon regulatory factor 1 (IRF-1) in the regulation of ornithine decarboxylase (ODC) gene expression during IFNγ macrophage activation
| Autor: | Lucia Malaguarnera, Livia Manzella, M. R. Pilastro, Angelo Messina, Maria Assunta Giuffrida, Giuseppe Girlando, Giuseppina Picardi |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Interferon-γ
Interferon regulatory factor 1 Molecular Sequence Data Biophysics Biology Biochemistry Gene Expression Regulation Enzymologic Cell Line Ornithine decarboxylase Interferon-gamma Structural Biology Cricetinae Gene expression Genetics Animals Humans Macrophage activation Molecular Biology Transcription factor Regulation of gene expression Base Sequence U937 cell DNA Cell Biology Transcription regulation Phosphoproteins Molecular biology DNA-Binding Proteins IRF1 IRF8 Interferon Regulatory Factor-1 Transcription Factors Interferon regulatory factors |
| Zdroj: | FEBS Letters. (2):177-180 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(94)00592-3 |
| Popis: | In this report we discuss the role of interferon regulatory factor 1 (IRF-1) in the regulation of ornithine decarboxylase (ODC) transcription during IFN gamma human macrophage activation. We show that a binding sequence for the transcription factor IRF-1 is contained in the first intron of the human ODC gene (from nt +2711 to nt +2722) and we demonstrate that the level of expression of IRF-1 increases in human macrophages and in the human promonocytic cell line, U937, previously differentiated in monocytes/macrophages by phorbol myristate acetate (PMA), after 2 h of IFN gamma stimulation. We also show that the hamster tk-ts13 cell line, stably transfected with the IRF-1 cDNA, over-expresses ODC. In addition, a specific complex was detected, by gel-shift assay after incubating a 20 bp double-stranded oligomer containing the binding sequence for IRF-1 with nuclear proteins extracted from human macrophages and from (PMA-differentiated) U937 cells stimulated with IFN gamma for 2 h. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|