A low-cost, rapidly integrated debubbler (RID) module for microfluidic cell culture applications

Autor: Nicole Mazzola, Nicholas K. Lee, Joseph A. Mylott, Adeel Ahmed, Matthew J. Williams, Vinay V. Abhyankar
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Micromachines, Vol 10, Iss 6, p 360 (2019)
Micromachines
Volume 10
Issue 6
DOI: 10.1101/629642
Popis: Microfluidic platforms use controlled fluid flows to provide physiologically relevant biochemical and biophysical cues to cultured cells in a well-defined and reproducible manner. Undisturbed flows are critical in these systems, and air bubbles entering microfluidic channels can lead to device delamination or cell damage. To prevent bubble entry into microfluidic channels, we report a low-cost, Rapidly Integrated Debubbler (RID) module that is simple to fabricate, inexpensive, and easily combined with existing experimental systems. We demonstrate successful removal of air bubbles spanning three orders of magnitude with a maximum removal rate (dV/dt)max = 1.5 mL min&minus
1, at flow rates required to apply physiological wall shear stress (1&ndash
200 dyne cm&minus
2) to mammalian cells cultured in microfluidic channels.
Databáze: OpenAIRE