A low-cost, rapidly integrated debubbler (RID) module for microfluidic cell culture applications
Autor: | Nicole Mazzola, Nicholas K. Lee, Joseph A. Mylott, Adeel Ahmed, Matthew J. Williams, Vinay V. Abhyankar |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Materials science
Orders of magnitude (temperature) lcsh:Mechanical engineering and machinery Bubble Microfluidics microfluidics 02 engineering and technology Wall shear Article 03 medical and health sciences Microfluidic channel Mammalian cell Shear stress Fluid dynamics lcsh:TJ1-1570 Electrical and Electronic Engineering 030304 developmental biology 0303 health sciences fluid-induced wall shear-stress (WSS) Mechanical Engineering mechanobiology 021001 nanoscience & nanotechnology Volumetric flow rate Control and Systems Engineering Cell culture 0210 nano-technology Biological system bubble removal |
Zdroj: | Micromachines, Vol 10, Iss 6, p 360 (2019) Micromachines Volume 10 Issue 6 |
DOI: | 10.1101/629642 |
Popis: | Microfluidic platforms use controlled fluid flows to provide physiologically relevant biochemical and biophysical cues to cultured cells in a well-defined and reproducible manner. Undisturbed flows are critical in these systems, and air bubbles entering microfluidic channels can lead to device delamination or cell damage. To prevent bubble entry into microfluidic channels, we report a low-cost, Rapidly Integrated Debubbler (RID) module that is simple to fabricate, inexpensive, and easily combined with existing experimental systems. We demonstrate successful removal of air bubbles spanning three orders of magnitude with a maximum removal rate (dV/dt)max = 1.5 mL min&minus 1, at flow rates required to apply physiological wall shear stress (1&ndash 200 dyne cm&minus 2) to mammalian cells cultured in microfluidic channels. |
Databáze: | OpenAIRE |
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