Characterisation of glycoconjugate sugar residues in the vomeronasal organ of the armadillo Chaetophractus villosus (Mammalia, xenarthra)
| Autor: | Jorge M. Affanni, Dante A. Paz, P. D. Carmanchahi, C. A. Sonez, C. C. Ferrari, H. J. Aldana Marcos |
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| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Male
Armadillos Histology Vomeronasal organ Glycoconjugate Otras Ciencias Biológicas Armadillo Biology Ciencias Biológicas purl.org/becyt/ford/1 [https] Vomeronasal receptor Olfactory mucosa Olfactory Mucosa biology.animal Lectins medicine Animals purl.org/becyt/ford/1.6 [https] Molecular Biology Ecology Evolution Behavior and Systematics chemistry.chemical_classification Binding Sites Mucous Membrane Histocytochemistry Differential staining Cell Biology Anatomy Molecular biology Mucus Epithelium Vomeronsal Organ medicine.anatomical_structure chemistry Female Vomeronasal Organ Glycoconjugates CIENCIAS NATURALES Y EXACTAS Research Article Developmental Biology |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| DOI: | 10.1046/j.1469-7580.2000.19630357.x |
| Popis: | Conventional carbohydrate histochemistry and the binding patterns of 21 lectins were analysed to characterise the glycoconjugate content in the components of the vomeronasal organ of the armadillo Chaetophractus villosus. The mucomicrovillous complex of the sensory epithelium bound most of the lectins studied. No reaction was observed with Con A, PSA, S‐Con A and SBA, and the sustentacular cells were stained with UEA‐I, DSL, LEL, STL and Con A. The vomeronasal receptor neurons were labelled with S‐WGA, WGA, PNA, UEA‐I, STL, Con A, S‐Con A, ECL and RCA120. The basal cell layer reacted with S‐WGA, WGA, LCA, UEA‐I, DSL, LEL, STL, Con A, JAC and VVA. The nonsensory epithelium exhibited a differential staining in relation to the different components. The mucociliary complex stained with ECL, DBA, JAC, RCA120, STL, LCA, PHA‐E, PHA‐L, LEL, BSL‐I and VVA. However, SJA and UEA‐I stained the mucus complex lining a subpopulation of columnar cells. The cytoplasm and cell membranes of columnar cells was labelled with DBA, DSL and LCA. The apical region of these cells exhibited moderate reactivity with LEL and SJA. None of the lectins bound specifically to secretory granules of the nonsecretory cells. Basal cells of the nonsensory epithelium were labelled with DSL, LEL, LCA, BSL‐I and STL. The vomeronasal glands showed a positive reaction with WGA, DSL, LEL, LCA, DBA, PNA, RCA120 and SBA. Subpopulations of acinar cells were observed with ECL, S‐WGA, Con A, S‐Con A and DBA. PNA and RCA120 stained the cells lining the glandular ducts. In comparison with previous results obtained in the olfactory mucosa of the same group of armadillos, the carbohydrate composition of the vomeronasal organ sensory epithelium differed from the olfactory sensory epithelium. This is probably related to the different nature of molecules involved in the perireceptor processes. Fil: Carmanchahi, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Ferrari, Carina Cintia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Aldana Marcos, Hernan Javier. Universidad de Morón; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Affanni, Jorge Mario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Soñez, Carlos Alberto. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina Fil: Paz, Dante Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina |
| Databáze: | OpenAIRE |
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