Advantages of multilocus sequence analysis for taxonomic studies: a case study using 10 housekeeping genes in the genus Ensifer (including former Sinorhizobium)
| Autor: | Renata Coopman, Monique Gillis, Paul De Vos, Miet Martens, Peter Dawyndt, Anne Willems |
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| Rok vydání: | 2008 |
| Předmět: |
Genetics
DNA Bacterial biology Sequence analysis Molecular Sequence Data Nucleic Acid Hybridization Genes rRNA General Medicine Sequence Analysis DNA Ribosomal RNA biology.organism_classification rpoB Microbiology Genetic analysis Housekeeping gene Bacterial Typing Techniques Bacterial Proteins Species Specificity 23S ribosomal RNA Genes Bacterial Rhizobiaceae Sinorhizobium RNA Ribosomal 16S Gene Ecology Evolution Behavior and Systematics |
| Zdroj: | International journal of systematic and evolutionary microbiology. 58(Pt 1) |
| ISSN: | 1466-5026 |
| Popis: | There is a need for easy, practical, reliable and robust techniques for the identification and classification of bacterial isolates to the species level as alternatives to 16S rRNA gene sequence analysis and DNA–DNA hybridization. Here, we demonstrate that multilocus sequence analysis (MLSA) of housekeeping genes is a valuable alternative technique. An MLSA study of 10 housekeeping genes (atpD, dnaK, gap, glnA, gltA, gyrB, pnp, recA, rpoB and thrC) was performed on 34 representatives of the genus Ensifer. Genetic analysis and comparison with 16S and 23S rRNA gene sequences demonstrated clear species boundaries and a higher discrimination potential for all housekeeping genes. Comparison of housekeeping gene sequence data with DNA–DNA reassociation data revealed good correlation at the intraspecies level, but indicated that housekeeping gene sequencing is superior to DNA–DNA hybridization for the assessment of genetic relatedness between Ensifer species. Our MLSA data, confirmed by DNA–DNA hybridizations, support the suggestion that Ensifer xinjiangensis is a later heterotypic synonym of Ensifer fredii. |
| Databáze: | OpenAIRE |
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