Myelin basic protein epitopes secreted by human T cells encounter natural autoantibodies in the serum
| Autor: | Gianni Zoccatelli, Anna Pia Riviera, G. Andrighetto, Elisabetta Stefani, Silvia Sartoris, Tiziana Cestari, Giuseppe Tridente, Roberto Chignola, C. Guerriero |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Epitopes
T-Lymphocyte Monoclonal Gammopathy of Undetermined Significance Epitope Epitopes Antibody Specificity T-Lymphocyte Subsets Immunology and Allergy Innate MBP epitopcs Child Cells Cultured Immunity Cellular Cultured biology Chemistry Peripheral tolerance Neurology Child Preschool thymocytes Antibody Multiple Sclerosis medicine.drug_class Immunoprecipitation Cells Immunology expression and secretion T lymphocytes natural autoantibodies Autoantibodies Binding Sites Antibody Humans Immune Sera Immunity Innate Immunoglobulin G Myelin Basic Protein Monoclonal antibody medicine Secretion Preschool Binding Sites Autoantibody Immunity Molecular biology Myelin basic protein T-Lymphocyte biology.protein Neurology (clinical) Cellular |
| Zdroj: | Università degli Studi di Verona-IRIS |
| Popis: | A previously isolated and characterized IgM monoclonal antibody (mAb 1H6.2) specific to myelin basic protein (MBP) and to MBP epitopes expressed by nonneural cells was used to immunoprecipitate and investigate the expression of MBP epitopes by human T cells. Peripheral T lymphocytes secreted MBP epitopes, and secretion increased in time after mitogen stimulation. Conversely, thymocytes secreted these proteins independently on mitogen stimulation. Specific antibody reactivity (primarily due to IgG3) towards immunoprecipitated MBP epitopes was found in all tested sera from healthy donors and from multiple sclerosis patients as well as in sera from normal human cord blood. Collectively, these data provide insights into the immunological mechanisms leading to central and peripheral tolerance to MBP products. |
| Databáze: | OpenAIRE |
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