Toxic waste disposal in Escherichia coli
| Autor: | Timothy H. Tran, Brian K. Janes, Robert B. Helling, Michael Bundesmann, Heather Kimball, Charles A. Miller, Darcy Phelan, Pietra Check |
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| Rok vydání: | 2002 |
| Předmět: |
Nalidixic acid
Lipoproteins Physiology and Metabolism Mutant medicine.disease_cause Microbiology Nalidixic Acid Bacterial Proteins Transcription (biology) medicine Escherichia coli Molecular Biology biology Membrane transport protein Escherichia coli Proteins Membrane Proteins Membrane Transport Proteins Drug Resistance Microbial biochemical phenomena metabolism and nutrition Biochemistry Mutation biology.protein DNA Transposable Elements bacteria Efflux Signal transduction Multidrug Resistance-Associated Proteins Sulfotransferases Carrier Proteins medicine.drug Bacterial Outer Membrane Proteins |
| Zdroj: | Journal of bacteriology. 184(13) |
| ISSN: | 0021-9193 |
| Popis: | About 10% of the nalidixic acid-resistant (Nal r ) mutants in a transposition-induced library exhibited a growth factor requirement as the result of cysH , icdA , metE , or purB mutation. Resistance in all of these mutants required a functional AcrAB-TolC efflux pump, but the EmrAB-TolC pump played no obvious role. Transcription of acrAB was increased in each type of Nal r mutant. In the icdA and purB mutants, each of the known signaling pathways appeared to be used in activating the AcrAB-TolC pump. The metabolites that accumulate upstream of the blocks caused by the mutations are hypothesized to increase the levels of the AcrAB-TolC pump, thereby removing nalidixic acid from the organism. |
| Databáze: | OpenAIRE |
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