| Autor: |
Mami Oba, Shoichi Sakaguchi, Hong Wu, Yoshihiko Fujioka, Hitoshi Takemae, Hisako Oki, Megumi Kawai, Mai Shiokawa, Hiroshi Aoki, Yuka Fukase, Hiroo Madarame, Takashi Nakano, Tetsuya Mizutani, Makoto Nagai |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
The Journal of general virology. 103(2) |
| ISSN: |
1465-2099 |
| Popis: |
A novel picornavirus was isolated from the faeces of a diarrhoeic cow using MA-104 cells at the third blind passage. This virus, named Den1/2021/JPN, was completely sequenced using total RNA from the cell culture supernatant by deep sequencing. The genome of Den1/2021/JPN had a standard picornavirus genome organisation with conserved picornaviral motifs. The 5′ untranslated region harboured a type-II internal ribosomal entry site. Den1/2021/JPN was most closely related to a bovine parechovirus (Bo_ParV) named cow/2018/4, which has been recently identified in publicly available databases. Phylogenetic analyses and pairwise sequence comparison revealed that Den1/2021/JPN and Bo_ParV cow/2018/4 clustered with parechoviruses and were most closely related to Parechovirus E identified in birds of prey, exhibiting nucleotide sequence similarity of 64.2–64.5 %, 58.6–59.7 % and 66.3–66.4 % in the polyprotein, P1 and 2C+3 CD coding regions, respectively. This study presents the first report on the isolation of Bo_ParV. Den1/2021/JPN and Bo_ParV cow/2018/4, which are candidates for a novel species in the genus Parechovirus. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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