The Loz1 transcription factor from Schizosaccharomyces pombe binds to Loz1 response elements and represses gene expression when zinc is in excess
| Autor: | Mark P. Foster, Stevin Wilson, Vibhuti Wadhwa, Amanda J. Bird, Yi-Hsuan Liu, Carlos Cardona-Soto |
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| Rok vydání: | 2019 |
| Předmět: |
Response element
Gene Expression Biology Response Elements Microbiology Article 03 medical and health sciences Gene Expression Regulation Fungal Schizosaccharomyces Gene expression Homeostasis Promoter Regions Genetic Cation Transport Proteins Molecular Biology Transcription factor 030304 developmental biology Regulation of gene expression Zinc finger 0303 health sciences 030306 microbiology Zinc Fingers Promoter biology.organism_classification Cell biology Zinc Regulon Schizosaccharomyces pombe Schizosaccharomyces pombe Proteins Transcription Factors |
| Zdroj: | Mol Microbiol |
| ISSN: | 1365-2958 0950-382X |
| DOI: | 10.1111/mmi.14384 |
| Popis: | In Schizosaccharomyces pombe, the expression of the zrt1 zinc uptake gene is tightly regulated by zinc status. When intracellular zinc levels are low, zrt1 is highly expressed. However, when zinc levels are high, transcription of zrt1 is blocked in a manner that is dependent upon the transcription factor Loz1. To gain additional insight into the mechanism by which Loz1 inhibits gene expression in high zinc, we used RNA-seq to identify Loz1-regulated genes, and ChIP-seq to analyze the recruitment of Loz1 to target gene promoters. We find that Loz1 is recruited to the promoters of 27 genes that are also repressed in high zinc in a Loz1-dependent manner. We also find that the recruitment of Loz1 to the majority of target gene promoters is dependent upon zinc and the motif 5'-CGN(A/C)GATCNTY-3', which we have named the Loz1 response element (LRE). Using reporter assays, we show that LREs are both required and sufficient for Loz1-mediated gene repression, and that the level of gene repression is dependent upon the number and sequence of LREs. Our results elucidate the Loz1 regulon in fission yeast and provide new insight into how eukaryotic cells are able to respond to changes in zinc availability in the environment. |
| Databáze: | OpenAIRE |
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