Bioanalytical method for quantification of Solifenacin in rat plasma by LC-MS/MS and its application to pharmacokinetic study
| Autor: | Chandrasekhar Kothapalli Bannoth, Rihana Parveen Shaik, Srinivasa Babu Puttagunta, Bahlul Z. Awen, Bala Sekhara Reddy Challa |
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| Jazyk: | angličtina |
| Předmět: |
Solifenacin Succinate
Bioanalysis Chromatography Solifenacin Chemistry Selected reaction monitoring General Physics and Astronomy General Chemistry Mass spectrometry General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Pharmacokinetics Ammonium formate medicine General Materials Science Methanol General Environmental Science medicine.drug |
| Zdroj: | Journal of Analytical Science and Technology. 5(1) |
| ISSN: | 2093-3371 |
| DOI: | 10.1186/s40543-014-0035-0 |
| Popis: | Background Solifenacin succinate is a competitive muscarinic acetylcholine receptor antagonist used in the treatment of overactive bladder with or without urge incontinence. Methods Liquid chromatography–tandem mass spectrometry method was used for quantification of Solifenacin (SF) in rat plasma. Solifenacin-d5 (SFD5) used as an internal standard. Chromatographic separation was performed Gemini-NX C18, 50 × 4.6 mm, 5 µm, 110 Å column. Mobile phase composed of 5 mM Ammonium formate, pH 3.0: methanol (20:80 v/v), with 0.4 mL/min flow-rate. Drug and IS were extracted by Liquid- liquid extraction method. SF and SFD5 were detected with proton adducts at m/z 363.2®193.2 and 368.2®198.2 in multiple reaction monitoring (MRM) positive mode respectively. The method was validated with the correlation coefficients of (r2) ≥ 0.9975 over a linear concentration range of 0.1-100.0 ng/mL. Results This method demonstrated intra and inter-day precision within 1.09 to 4.84 and 1.75 to 7.68 % and accuracy within 101.21 to 106.67 and 97.94 to 104.79 % for SF. Conclusions This method is successfully applied in the Pharmacokinetic study of rat plasma. |
| Databáze: | OpenAIRE |
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