Complete genome sequence of a phage hyperparasite of Candidatus Xenohaliotis californiensis (Rickettsiales) – a pathogen of Haliotis spp (Gasteropoda)
| Autor: | Alexei F. Licea-Navarro, Ricardo Gonzales-Sánchez, Abraham Guerrero, Jorge Cáceres-Martínez, Miguel Ángel Del Río-Portilla, Roberto Cruz-Flores |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Haliotis rufescens Gastropoda 030106 microbiology Gene Expression Genome Viral Genome Open Reading Frames 03 medical and health sciences Virology Animals Bacteriophages ORFS RNA Double-Stranded Whole genome sequencing Genetics Hyperparasite Whole Genome Sequencing biology Nucleotide transport Chromosome Mapping Haliotis corrugata General Medicine biology.organism_classification Anaplasmataceae 030104 developmental biology Biological Control Agents RNA Viral Rickettsiales |
| Zdroj: | Archives of Virology. 163:1101-1104 |
| ISSN: | 1432-8798 0304-8608 |
| Popis: | Bacteriophages are recognized as major mortality agents of microbes, among them intracellular marine rickettsiales-like bacteria. Recently, a phage hyperparasite of Candidatus Xenohaliotis californiensis (CXc) has been described. This bacterium is considered the causal agent of Withering Syndrome (WS) which is a chronic and potentially lethal disease of abalone species from California, USA and the peninsula of Baja California, Mexico. This hyperparasite which infects CXc could be used as a biocontrol agent for WS. Therefore, it is necessary to obtain genomic information to characterize this phage. In this study, the first complete genome sequence of a novel phage, Xenohaliotis phage (pCXc) was determined. The complete genome of pCXc from red abalone (Haliotis rufescens) is 35,728 bp, while the complete genome of pCXc from yellow abalone (Haliotis corrugata) is 35,736 bp. Both phage genomes consist of double-stranded DNA with a G + C content of 38.9%. In both genomes 33 open reading frames (ORFs) were predicted. Only 10 ORFs encode proteins that have identifiable functional homologues. These 10 ORFs were classified by function, including structural, DNA replication, DNA packaging, nucleotide transport and metabolism, life cycle regulation, recombination and repair, and additional functions. A PCR method for the specific detection of pCXc was developed. This information will help to understand a new group of phages that infect intracellular marine rickettsiales-like bacteria in mollusks. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink