Role of S-methylisothiourea (SMT) in renal ischemia/reperfusion injury in rats
| Autor: | Fatemeh Eshraghi-Jazi, Fatemeh Kanani, Ardeshir Talebi, Mohaddeseh Mojarradfard, Fatemeh Moslemi, Faezeh Fazelnia, Mehdi Nematbakhsh |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
Urology medicine.medical_treatment 030232 urology & nephrology Renal function 030204 cardiovascular system & hematology urologic and male genital diseases Nitric oxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Renal injury Saline Blood urea nitrogen Kidney Creatinine Renal ischemia reperfusion biology Renal ischemia urogenital system business.industry Nitric oxide synthase medicine.anatomical_structure chemistry Nephrology Anesthesia biology.protein Original Article S-methylisothiourea business |
| Zdroj: | Journal of Renal Injury Prevention |
| ISSN: | 2345-2781 |
| DOI: | 10.15171/jrip.2016.07 |
| Popis: | Introduction: Excessive production of nitric oxide (NO) via inducible nitric oxide synthase (iNOS) is associated in renal ischemia reperfusion injury (IRI). Objectives: This study was designed to investigate the role of S-methylisothiourea (SMT) as selective inhibitor iNOS in renal IRI. Materials and Methods: Male Wistar rats were subjected to 45 minutes of bilateral renal ischemia by occlusion of renal vessels of both kidney followed by 24 hours of reperfusion. Prior to renal IRI, the rats received either vehicle (saline, group 2) or SMT (50 mg/kg, group 3), and were compared with the sham-operated animals (group 1). At the end of reperfusion period, the rats were sacrificed for kidney tissue pathology investigation. Results: Serum creatinine (Cr), blood urea nitrogen (BUN), nitrite levels, and kidney weight significantly increased in groups 2 and 3 (P < 0.05). Kidney tissue damage scores in groups 2 and 3 were also higher than that in the sham-operated group (P < 0.05). Conclusion: SMT not only prevent the kidney during IRI, but also promotes kidney function disturbance and severity of renal injury. |
| Databáze: | OpenAIRE |
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