Bactericidal effect of hydroxyl radicals generated from a low concentration hydrogen peroxide with ultrasound in endodontic treatment
| Autor: | Masaichi-Chang-il Lee, Yoshimi Kobayashi, Fumihiko Yoshino, Makoto Hayashi, Kuniyasu Ochiai, Haruna Ibi, Muneaki Tamura, Ayaka Yoshida, Bunnai Ogiso |
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| Rok vydání: | 2014 |
| Předmět: |
Sodium
Radical Clinical Biochemistry Medicine (miscellaneous) chemistry.chemical_element hydrogen peroxide Nanotechnology Photochemistry Enterococcus faecalis law.invention chemistry.chemical_compound law Irradiation Hydrogen peroxide Electron paramagnetic resonance chemistry.chemical_classification Reactive oxygen species Nutrition and Dietetics hydroxyl radical biology ultrasound electron spin resonance bactericidal effect biology.organism_classification chemistry Original Article Hydroxyl radical |
| Zdroj: | Journal of Clinical Biochemistry and Nutrition |
| ISSN: | 1880-5086 0912-0009 |
| DOI: | 10.3164/jcbn.13-86 |
| Popis: | One approach to enhance the disinfection of root canals in endodontic treatment is ultrasonic irrigation with sodium hypochlorite. Reactive oxygen species, such as hydroxyl radical, are generated by biological defense systems to kill invading bacteria. Ultrasonic irrigation with hydrogen peroxide may be a promising option to increase hydroxyl radical generation. We examined the bactericidal effects of hydroxyl radical generated from low concentration hydrogen peroxide with ultrasound in vitro. An ultrasonic tip was submerged in 0.5 or 1.0 M hydrogen peroxide in a microfuge tube. hydrogen peroxide was irradiated with the ultrasound, the tip of which was maintained centered in the tube to mimic ultrasonic irrigation. Hydroxyl radical generation was assessed by electron spin resonance spectroscopy. Subsequently, Enterococcus faecalis suspension in hydrogen peroxide was prepared and irradiated as described above. Bactericidal effects were assessed by viable counting. Electron spin resonance measurements showed that hydroxyl radical generation increased significantly in a time- and dose-dependent manner (two-way analysis of variance and Tukey’s test, p |
| Databáze: | OpenAIRE |
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