Abnormalities of the p53 tumour suppressor gene in human pancreatic cancer
| Autor: | S. L. Staddon, R. C. G. Russell, Christine M. Hughes, C. M. Barton, Peter A. Hall, G. Klöppel, Robin C. N. Williamson, B. Theis, John P. Neoptolemos, C. O'Sullivan |
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| Rok vydání: | 1991 |
| Předmět: |
Cancer Research
Tumor suppressor gene Blotting Western DNA Mutational Analysis Molecular Sequence Data Mutant Biology medicine.disease_cause Pancreatic cancer Tumor Cells Cultured medicine Humans Genes Tumor Suppressor Mutation Base Sequence Antibodies Monoclonal Genes p53 medicine.disease Precipitin Tests Pancreatic Neoplasms medicine.anatomical_structure Genes Oligodeoxyribonucleotides Oncology Cancer research Immunohistochemistry CA19-9 Tumor Suppressor Protein p53 Carcinogenesis Pancreas Research Article |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1038/bjc.1991.467 |
| Popis: | The tumour suppressor gene p53 has been found to be mutated or inactivated at high frequency in several common human tumours. We have examined a series of exocrine pancreatic carcinomas for over-expression of mutant forms of p53 by immunohistochemistry with a panel of specific antibodies. We found immunodetectable p53 in 13 of 22 (60%) frozen pancreatic cancers and seven of 13 pancreatic cell lines. One of the antibodies, CM1, recognises p53 in formalin-fixed, paraffin-embedded archival material and using this reagent we found immunodetectable p53 in 28 of 124 (23%) pancreatic cancers. We have successfully demonstrated the presence of point mutations by direct sequencing of genomic DNA extracted from archival tissue showing CM1 immunoreactivity. We conclude that p53 activation is an important event in human pancreatic tumorigenesis and that the CM1 antibody can detect a proportion of cases of overexpression of mutant p53 in archival pathological material. Images Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 |
| Databáze: | OpenAIRE |
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