Sinobacterium norvegicum sp . nov., a new species isolated from scallop (Pecten maximus)

Autor: Dieguez Ana Belen, Jacobsen Anita, Romalde Jesus, Magnesen Throlf
Rok vydání: 2014
Předmět:
Zdroj: Frontiers in Marine Science, Vol 1 (2014)
ISSN: 2296-7745
DOI: 10.3389/conf.fmars.2014.02.00118
Popis: The family Oceanospirillaceae (Garrity et al., 2005) includes 18 genera, 11 of which were described in the last 10 years (Arahal et al., 2007). Members of this family are motile and exclusive from aquatic environments. As part of a study on the microbiota associated to Great scallop (Pecten maximus) and its rearing system performed in a hatchery in Bergen (Norway), seven strains: 3CM4T(= CECT 8267T; = CAIM 1884T) 3CH4, 2CH8, 2SH3, 1CH5, 1CH6 and 1SH4 (= CECT 8268; = CAIM 1885) were isolated from broodstock gonads. These strains are aerobic, oxidase and catalase positive and they do not grow in TCBS. Isolates form small (less than 1 mm) pale brown pigmentated colonies on marine agar. They are halophiles and their temperature range for growth is 4 to 20ºC. Cells are Gram-negative, non-motile long rods, measuring 0.2-0.4 μm in diameter and 5-20 μm in length. With oxidative metabolism, the isolates do not ferment carbohydrates. The results of analysis of DNA-DNA hybridization confirmed that these isolates constitute a new and differentiated cluster within the genus Sinobacterium. The predominant fatty acids were C16: 0, C16: 1 ω7c/C16: 1 ω6c and C18: 1 ω7c. The G + C content of the new isolates is 52.2 ± 1 mol%. Based on phenotypic and phylogenetic characteristics, the seven isolates represent a new species for which the name Sinobacterium norvegicum sp. nov. is proposed, with strain 3CM4T (= CECT 8267T = CAIM 1884T) as the type strain.
Databáze: OpenAIRE