βPIX and GIT1 regulate HGF-induced lamellipodia formation and WAVE2 transport
| Autor: | Shigeru Morimura, Kazuhide Takahashi, Katsuo Suzuki |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Cell
Biophysics Cell Cycle Proteins macromolecular substances Biology Biochemistry Cell Movement RNA interference Cell Line Tumor medicine Guanine Nucleotide Exchange Factors Humans Pseudopodia Molecular Biology Actin Adaptor Proteins Signal Transducing Hepatocyte Growth Factor Cell migration Cell Biology Membrane transport Wiskott-Aldrich Syndrome Protein Family Cell biology Protein Transport medicine.anatomical_structure Hepatocyte growth factor Lamellipodium Human breast Rho Guanine Nucleotide Exchange Factors medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 382:614-619 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2009.03.083 |
| Popis: | Formation of lamellipodia is the first step during cell migration, and involves actin reassembly at the leading edge of migrating cells through the membrane transport of WAVE2. However, the factors that regulate WAVE2 transport to the cell periphery for initiating lamellipodia formation have not been elucidated. We report here that in human breast cancer MDA-MB-231 cells, the hepatocyte growth factor (HGF) induced the association between the constitutive complex of βPIX and GIT1 with WAVE2, which was concomitant with the induction of lamellipodia formation and WAVE2 transport. Although depletion of βPIX by RNA interference abrogated the HGF-induced WAVE2 transport and lamellipodia formation, GIT1 depletion caused HGF-independent WAVE2 transport and lamellipodia formation. Collectively, we suggest that βPIX releases cells from the GIT1-mediated suppression of HGF-independent responses and recruits GIT1 to WAVE2, thereby facilitating HGF-induced WAVE2 transport and lamellipodia formation. |
| Databáze: | OpenAIRE |
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