A novel epitope of N-CAM defines precursors of human adherent NK cells
| Autor: | Valeria P. Makarenkova, William H. Chambers, Ronald B. Herberman, William E. Gooding, Tjendimin Tjandrawan, Shen Li, Jun Xu, Theresa L. Whiteside, Jukka Vakkila, Torsten E. Reichert, Carl F. Lagenaur, Nikola L. Vujanovic, Cristian L. Achim |
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| Rok vydání: | 2004 |
| Předmět: |
Cytotoxicity
Immunologic Integrins Lymphoid Tissue Immunology Cell Count Biology CD49b Immunophenotyping Epitopes Interleukin 21 NK-92 Cell Adhesion Humans Immunology and Allergy Cell Lineage Lectins C-Type Antigen-presenting cell Cells Cultured Cell Proliferation Membrane Glycoproteins Lymphokine-activated killer cell Stem Cells Janus kinase 3 Cell Membrane Receptors IgG Antibodies Monoclonal Cell Differentiation Cell Biology Natural killer T cell CD56 Antigen Cell biology Killer Cells Natural Chemotaxis Leukocyte Antigens Surface Interleukin 12 Interleukin-2 NK Cell Lectin-Like Receptor Subfamily B |
| Zdroj: | Journal of Leukocyte Biology. 76:1187-1199 |
| ISSN: | 1938-3673 0741-5400 |
| DOI: | 10.1189/jlb.0802386 |
| Popis: | Activated, adherent natural killer (A-NK) cells represent a distinct subpopulation of interleukin (IL)-2-stimulated NK cells, which are selectively endowed with the increased expression of integrins and ability to adhere to solid surfaces, migrate into, infiltrate, and destroy cancerous tissues. The present study defines the phenotype and functions of precursors of A-NK (pre-A-NK) cells in humans. Peripheral blood pre-A-NK cells, in contrast to the rest of NK cells, express a novel epitope of CD56 neuronal cell adhesion molecule, termed ANK-1, and increased cell-surface levels of integrins. Pre-A-NK cells also express low levels of CD56 and CD161, and some express CD162 receptor, do not express CD25 or activation markers, and are effective mediators of NK cytotoxicity. Thus, pre-A-NK cells are generally similar to CD56dim NK cells. However, pre-A-NK cells differ from the main NK cell subpopulation by having a lower expression level of CD16 and a lower ability to mediate redirected antibody-dependent, cell-mediated cytotoxicity. More importantly, pre-A-NK cells are preferentially endowed with the ability to rapidly respond to IL-2 by integrin-mediated adherence to endothelial cells, extracellular matrix, and plastic. This early, specific response of pre-A-NK cells to IL-2 is followed by their activation, vigorous proliferation, and differentiation into phenotypically and functionally similar A-NK cells. Pre-A-NK cells represent only ∼26% of peripheral blood NK cells but encompass the majority of NK cells in normal and cancerous, solid tissues. We conclude that pre-A-NK cells represent a distinct subset of resting, mature NK cells with the characteristics indicative of their ability to migrate and reside in solid tissues. |
| Databáze: | OpenAIRE |
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