Optical control of NMDA-receptors with a diffusible photoswitch
| Autor: | Dirk Trauner, Michael Hollmann, Laura Laprell, Martin Sumser, Jan Terhag, Vilius Franckevicius, Felix W. W. Hartrampf, Nelson Rebola, Emilienne Repak, David A. DiGregorio |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Agonist
Patch-Clamp Techniques Light medicine.drug_class Ultraviolet Rays General Physics and Astronomy Glutamic Acid Kainate receptor Biology Hippocampus Receptors N-Methyl-D-Aspartate General Biochemistry Genetics and Molecular Biology Article Mice Xenopus laevis medicine Animals Protein Isoforms Long-term depression CA1 Region Hippocampal Cerebral Cortex Multidisciplinary Photoswitch Pyramidal Cells Glutamate receptor General Chemistry Glutamic acid Rats Biochemistry nervous system Synaptic plasticity Biophysics Oocytes NMDA receptor |
| Zdroj: | Nature Communications |
| DOI: | 10.1038/ncomms9076 |
| Popis: | N-methyl-D-aspartate receptors (NMDARs) play a central role in synaptic plasticity, learning and memory, and are implicated in various neuronal disorders. We synthesized a diffusible photochromic glutamate analogue, azobenzene-triazole-glutamate (ATG), which is specific for NMDARs and functions as a photoswitchable agonist. ATG is inactive in its dark-adapted trans-isoform, but can be converted into its active cis-isoform using one-photon (near UV) or two-photon (740 nm) excitation. Irradiation with violet light photo-inactivates ATG within milliseconds, allowing agonist removal on the timescale of NMDAR deactivation. ATG is compatible with Ca2+ imaging and can be used to optically mimic synaptic coincidence detection protocols. Thus, ATG can be used like traditional caged glutamate compounds, but with the added advantages of NMDAR specificity, low antagonism of GABAR-mediated currents, and precise temporal control of agonist delivery. N-methyl-D-aspartate receptors (NMDARs) play a central role in synaptic plasticity, learning and memory. Here the authors describe azobenzene-triazole-glutamate (ATG), a new diffusible photoswitchable agonist that allows precise temporal control over NMDAR activity. |
| Databáze: | OpenAIRE |
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